Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACDW36_RS28370 Genome accession   NZ_CP167984
Coordinates   6087618..6088241 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 0304d2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6082618..6093241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW36_RS28350 (ACDW36_28350) aroB 6083213..6084319 (-) 1107 WP_023096238.1 3-dehydroquinate synthase -
  ACDW36_RS28355 (ACDW36_28355) aroK 6084369..6084887 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACDW36_RS28360 (ACDW36_28360) pilQ 6084899..6087043 (-) 2145 WP_372437005.1 type 4a pilus secretin PilQ Machinery gene
  ACDW36_RS28365 (ACDW36_28365) pilP 6087097..6087621 (-) 525 WP_372437006.1 type 4a pilus biogenesis lipoprotein PilP -
  ACDW36_RS28370 (ACDW36_28370) pilO 6087618..6088241 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACDW36_RS28375 (ACDW36_28375) pilN 6088238..6088834 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACDW36_RS28380 (ACDW36_28380) pilM 6088834..6089898 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACDW36_RS28385 (ACDW36_28385) - 6090083..6092554 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=933211 ACDW36_RS28370 WP_003103268.1 6087618..6088241(-) (pilO) [Pseudomonas aeruginosa strain 0304d2]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=933211 ACDW36_RS28370 WP_003103268.1 6087618..6088241(-) (pilO) [Pseudomonas aeruginosa strain 0304d2]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAACCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401