Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACDW36_RS28375 Genome accession   NZ_CP167984
Coordinates   6088238..6088834 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain 0304d2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6083238..6093834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW36_RS28355 (ACDW36_28355) aroK 6084369..6084887 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACDW36_RS28360 (ACDW36_28360) pilQ 6084899..6087043 (-) 2145 WP_372437005.1 type 4a pilus secretin PilQ Machinery gene
  ACDW36_RS28365 (ACDW36_28365) pilP 6087097..6087621 (-) 525 WP_372437006.1 type 4a pilus biogenesis lipoprotein PilP -
  ACDW36_RS28370 (ACDW36_28370) pilO 6087618..6088241 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACDW36_RS28375 (ACDW36_28375) pilN 6088238..6088834 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACDW36_RS28380 (ACDW36_28380) pilM 6088834..6089898 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACDW36_RS28385 (ACDW36_28385) - 6090083..6092554 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=933212 ACDW36_RS28375 WP_003095839.1 6088238..6088834(-) (pilN) [Pseudomonas aeruginosa strain 0304d2]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=933212 ACDW36_RS28375 WP_003095839.1 6088238..6088834(-) (pilN) [Pseudomonas aeruginosa strain 0304d2]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369