Detailed information NTG0932949

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3O05_RS14255
• Genome accession: NZ_CP144249
• Coordinates: 2991281..2992399 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain YFY24
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2986281..2997399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3O05_RS14245 (V3O05_14245)	-	2989397..2990089 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
V3O05_RS14250 (V3O05_14250)	pilT	2990216..2991253 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
V3O05_RS14255 (V3O05_14255)	pilU	2991281..2992399 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V3O05_RS14260 (V3O05_14260)	fur	2992469..2992906 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
V3O05_RS14265 (V3O05_14265)	bamE	2993018..2993416 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
V3O05_RS14270 (V3O05_14270)	-	2993471..2993794 (-)	324	WP_000442587.1	RnfH family protein	-
V3O05_RS14275 (V3O05_14275)	-	2993794..2994861 (-)	1068	WP_002019572.1	hypothetical protein	-
V3O05_RS14280 (V3O05_14280)	-	2995014..2995469 (-)	456	WP_000782976.1	bacteriohemerythrin	-
V3O05_RS14285 (V3O05_14285)	-	2995722..2996570 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=932949 V3O05_RS14255 WP_000347039.1 2991281..2992399(+) (pilU) [Acinetobacter baumannii strain YFY24]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=932949 V3O05_RS14255 WP_000347039.1 2991281..2992399(+) (pilU) [Acinetobacter baumannii strain YFY24]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395