Detailed information NTG0932902

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3O06_RS14370
• Genome accession: NZ_CP144247
• Coordinates: 3012426..3013544 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain YFY21
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3007426..3018544

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3O06_RS14360 (V3O06_14365)	-	3010542..3011234 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
V3O06_RS14365 (V3O06_14370)	pilT	3011361..3012398 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
V3O06_RS14370 (V3O06_14375)	pilU	3012426..3013544 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V3O06_RS14375 (V3O06_14380)	fur	3013614..3014051 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
V3O06_RS14380 (V3O06_14385)	bamE	3014163..3014561 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
V3O06_RS14385 (V3O06_14390)	-	3014616..3014939 (-)	324	WP_000442587.1	RnfH family protein	-
V3O06_RS14390 (V3O06_14395)	-	3014939..3016006 (-)	1068	WP_002019572.1	hypothetical protein	-
V3O06_RS14395 (V3O06_14400)	-	3016159..3016614 (-)	456	WP_000782976.1	bacteriohemerythrin	-
V3O06_RS14400 (V3O06_14405)	-	3016867..3017715 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=932902 V3O06_RS14370 WP_000347039.1 3012426..3013544(+) (pilU) [Acinetobacter baumannii strain YFY21]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=932902 V3O06_RS14370 WP_000347039.1 3012426..3013544(+) (pilU) [Acinetobacter baumannii strain YFY21]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395