Detailed information NTG0932852

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3O04_RS14255
• Genome accession: NZ_CP144242
• Coordinates: 2980921..2982039 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain SRM3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2975921..2987039

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3O04_RS14245 (V3O04_14240)	-	2979037..2979729 (-)	693	WP_002124845.1	YggS family pyridoxal phosphate-dependent enzyme	-
V3O04_RS14250 (V3O04_14245)	pilT	2979856..2980893 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
V3O04_RS14255 (V3O04_14250)	pilU	2980921..2982039 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V3O04_RS14260 (V3O04_14255)	fur	2982109..2982546 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
V3O04_RS14265 (V3O04_14260)	bamE	2982658..2983056 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
V3O04_RS14270 (V3O04_14265)	-	2983111..2983434 (-)	324	WP_000442589.1	RnfH family protein	-
V3O04_RS14275 (V3O04_14270)	-	2983434..2984501 (-)	1068	WP_004835750.1	hypothetical protein	-
V3O04_RS14280 (V3O04_14275)	-	2984654..2985109 (-)	456	WP_029574892.1	bacteriohemerythrin	-
V3O04_RS14285 (V3O04_14280)	-	2985362..2986210 (-)	849	WP_029574891.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=932852 V3O04_RS14255 WP_000347036.1 2980921..2982039(+) (pilU) [Acinetobacter baumannii strain SRM3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=932852 V3O04_RS14255 WP_000347036.1 2980921..2982039(+) (pilU) [Acinetobacter baumannii strain SRM3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392