Detailed information NTG0932795

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3O01_RS04575
• Genome accession: NZ_CP144240
• Coordinates: 966178..967296 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain SRM25
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 961178..972296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3O01_RS04545 (V3O01_04545)	-	962007..962855 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
V3O01_RS04550 (V3O01_04550)	-	963108..963563 (+)	456	WP_000782976.1	bacteriohemerythrin	-
V3O01_RS04555 (V3O01_04555)	-	963716..964783 (+)	1068	WP_002019572.1	hypothetical protein	-
V3O01_RS04560 (V3O01_04560)	-	964783..965106 (+)	324	WP_000442587.1	RnfH family protein	-
V3O01_RS04565 (V3O01_04565)	bamE	965161..965559 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
V3O01_RS04570 (V3O01_04570)	fur	965671..966108 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
V3O01_RS04575 (V3O01_04575)	pilU	966178..967296 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V3O01_RS04580 (V3O01_04580)	pilT	967324..968361 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
V3O01_RS04585 (V3O01_04585)	-	968488..969180 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=932795 V3O01_RS04575 WP_000347039.1 966178..967296(-) (pilU) [Acinetobacter baumannii strain SRM25]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=932795 V3O01_RS04575 WP_000347039.1 966178..967296(-) (pilU) [Acinetobacter baumannii strain SRM25]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395