Detailed information NTG0932004

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: ACB328_RS00345
• Genome accession: NZ_CP167123
• Coordinates: 54230..55228 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain Isolate 32
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49230..60228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACB328_RS00325 (ACB328_00325)	-	50008..51651 (+)	1644	WP_010921779.1	AIPR family protein	-
ACB328_RS00330 (ACB328_00330)	purB	51669..52961 (+)	1293	WP_010921780.1	adenylosuccinate lyase	-
ACB328_RS00335 (ACB328_00335)	comR	53093..54004 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
ACB328_RS00340 (ACB328_00340)	comS	54088..54183 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
ACB328_RS00345 (ACB328_00345)	ruvB	54230..55228 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
ACB328_RS00350 (ACB328_00350)	-	55366..55803 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
ACB328_RS00355 (ACB328_00355)	-	55826..56227 (+)	402	WP_010921781.1	MORN repeat-containing protein	-
ACB328_RS00360 (ACB328_00360)	-	56224..57999 (+)	1776	WP_010921782.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=932004 ACB328_RS00345 WP_002986679.1 54230..55228(+) (ruvB) [Streptococcus pyogenes strain Isolate 32]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=932004 ACB328_RS00345 WP_002986679.1 54230..55228(+) (ruvB) [Streptococcus pyogenes strain Isolate 32]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473