Detailed information NTG0931959

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2191_RS09330
• Genome accession: NZ_CP143907
• Coordinates: 2045257..2046405 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2040257..2051405

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2191_RS09305 (V2191_09305)	-	2041408..2041704 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
V2191_RS09310 (V2191_09310)	-	2041701..2042294 (-)	594	WP_003084544.1	YggT family protein	-
V2191_RS09315 (V2191_09315)	proC	2042305..2043126 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
V2191_RS09320 (V2191_09320)	-	2043138..2043830 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2191_RS09325 (V2191_09325)	pilT	2044045..2045079 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2191_RS09330 (V2191_09330)	pilU	2045257..2046405 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
V2191_RS09335 (V2191_09335)	czcD	2046413..2047312 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2191_RS09340 (V2191_09340)	-	2047467..2047871 (+)	405	WP_003084560.1	NINE protein	-
V2191_RS09345 (V2191_09345)	-	2048114..2049487 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V2191_RS09350 (V2191_09350)	-	2049484..2050668 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=931959 V2191_RS09330 WP_003100959.1 2045257..2046405(+) (pilU) [Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931959 V2191_RS09330 WP_003100959.1 2045257..2046405(+) (pilU) [Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382