Detailed information NTG0931857

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2197_RS10585
• Genome accession: NZ_CP143901
• Coordinates: 2230743..2231891 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PA12_L1_25.22_ST207
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2225743..2236891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2197_RS10565 (V2197_10565)	-	2226480..2227664 (-)	1185	WP_023092310.1	cystathionine gamma-synthase	-
V2197_RS10570 (V2197_10570)	-	2227661..2229034 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V2197_RS10575 (V2197_10575)	-	2229277..2229681 (-)	405	WP_003084560.1	NINE protein	-
V2197_RS10580 (V2197_10580)	czcD	2229836..2230735 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2197_RS10585 (V2197_10585)	pilU	2230743..2231891 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
V2197_RS10590 (V2197_10590)	pilT	2232069..2233103 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2197_RS10595 (V2197_10595)	-	2233318..2234010 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2197_RS10600 (V2197_10600)	proC	2234022..2234843 (+)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
V2197_RS10605 (V2197_10605)	-	2234854..2235447 (+)	594	WP_003084544.1	YggT family protein	-
V2197_RS10610 (V2197_10610)	-	2235444..2235740 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=931857 V2197_RS10585 WP_003110016.1 2230743..2231891(-) (pilU) [Pseudomonas aeruginosa strain PA12_L1_25.22_ST207]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931857 V2197_RS10585 WP_003110016.1 2230743..2231891(-) (pilU) [Pseudomonas aeruginosa strain PA12_L1_25.22_ST207]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382