Detailed information NTG0931764

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2200_RS15985
• Genome accession: NZ_CP143898
• Coordinates: 3392399..3393547 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA11_WM3_21.22_ST27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3387399..3398547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2200_RS15960 (V2200_15960)	-	3388550..3388846 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
V2200_RS15965 (V2200_15965)	-	3388843..3389436 (-)	594	WP_003084544.1	YggT family protein	-
V2200_RS15970 (V2200_15970)	proC	3389447..3390268 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
V2200_RS15975 (V2200_15975)	-	3390280..3390972 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2200_RS15980 (V2200_15980)	pilT	3391187..3392221 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2200_RS15985 (V2200_15985)	pilU	3392399..3393547 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
V2200_RS15990 (V2200_15990)	czcD	3393555..3394454 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2200_RS15995 (V2200_15995)	-	3394608..3395012 (+)	405	WP_003084560.1	NINE protein	-
V2200_RS16000 (V2200_16000)	-	3395255..3396628 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
V2200_RS16005 (V2200_16005)	-	3396625..3397809 (+)	1185	WP_023114752.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=931764 V2200_RS15985 WP_003100959.1 3392399..3393547(+) (pilU) [Pseudomonas aeruginosa strain PA11_WM3_21.22_ST27]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931764 V2200_RS15985 WP_003100959.1 3392399..3393547(+) (pilU) [Pseudomonas aeruginosa strain PA11_WM3_21.22_ST27]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382