Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   VP472_RS12360 Genome accession   NZ_CP143632
Coordinates   2539831..2540445 (-) Length   204 a.a.
NCBI ID   WP_146277193.1    Uniprot ID   -
Organism   Bacillus sp. MT(2024)     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2534831..2545445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP472_RS12330 (VP472_12330) rpsT 2535131..2535397 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  VP472_RS12335 (VP472_12335) holA 2535414..2536455 (-) 1042 Protein_2382 DNA polymerase III subunit delta -
  VP472_RS12340 (VP472_12340) - 2536495..2536649 (-) 155 Protein_2383 hypothetical protein -
  VP472_RS12345 (VP472_12345) - 2536690..2536824 (+) 135 WP_003152870.1 YqzM family protein -
  VP472_RS12350 (VP472_12350) comEC 2536843..2539194 (-) 2352 WP_146277191.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  VP472_RS12355 (VP472_12355) - 2539195..2539764 (-) 570 WP_003152868.1 ComE operon protein 2 -
  VP472_RS12360 (VP472_12360) comEA 2539831..2540445 (-) 615 WP_146277193.1 helix-hairpin-helix domain-containing protein Machinery gene
  VP472_RS12365 (VP472_12365) comER 2540504..2541325 (+) 822 WP_146277195.1 late competence protein ComER -
  VP472_RS12370 (VP472_12370) - 2541394..2542131 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  VP472_RS12375 (VP472_12375) rsfS 2542128..2542484 (-) 357 WP_003152864.1 ribosome silencing factor -
  VP472_RS12380 (VP472_12380) yqeK 2542502..2543062 (-) 561 WP_032856887.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  VP472_RS12385 (VP472_12385) - 2543052..2543621 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  VP472_RS12390 (VP472_12390) yhbY 2543632..2543922 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  VP472_RS12395 (VP472_12395) aroE 2543916..2544758 (-) 843 WP_015417858.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 6.0523

>NTDB_id=930047 VP472_RS12360 WP_146277193.1 2539831..2540445(-) (comEA) [Bacillus sp. MT(2024)]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLRDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=930047 VP472_RS12360 WP_146277193.1 2539831..2540445(-) (comEA) [Bacillus sp. MT(2024)]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCGGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.333

94.118

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363