Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VP472_RS11935 Genome accession   NZ_CP143632
Coordinates   2463322..2464392 (-) Length   356 a.a.
NCBI ID   WP_070082113.1    Uniprot ID   -
Organism   Bacillus sp. MT(2024)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458322..2469392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP472_RS11885 (VP472_11885) - 2458382..2458966 (-) 585 WP_012117977.1 signal peptidase I -
  VP472_RS11890 (VP472_11890) tapA 2458938..2459609 (-) 672 WP_070082109.1 amyloid fiber anchoring/assembly protein TapA -
  VP472_RS11895 (VP472_11895) - 2459868..2460197 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  VP472_RS11900 (VP472_11900) - 2460237..2460416 (-) 180 WP_003153093.1 YqzE family protein -
  VP472_RS11905 (VP472_11905) comGG 2460473..2460850 (-) 378 WP_094247734.1 competence type IV pilus minor pilin ComGG Machinery gene
  VP472_RS11910 (VP472_11910) comGF 2460851..2461246 (-) 396 WP_094247733.1 competence type IV pilus minor pilin ComGF -
  VP472_RS11915 (VP472_11915) comGE 2461260..2461574 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  VP472_RS11920 (VP472_11920) comGD 2461558..2461995 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  VP472_RS11925 (VP472_11925) comGC 2461985..2462293 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  VP472_RS11930 (VP472_11930) comGB 2462298..2463335 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  VP472_RS11935 (VP472_11935) comGA 2463322..2464392 (-) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  VP472_RS11940 (VP472_11940) - 2464584..2465534 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  VP472_RS11945 (VP472_11945) - 2465680..2466981 (+) 1302 WP_070082114.1 hemolysin family protein -
  VP472_RS11950 (VP472_11950) - 2467019..2467849 (-) 831 WP_070082115.1 STAS domain-containing protein -
  VP472_RS11960 (VP472_11960) - 2468055..2468435 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  VP472_RS11965 (VP472_11965) - 2468672..2468917 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.91 Da        Isoelectric Point: 9.5862

>NTDB_id=930043 VP472_RS11935 WP_070082113.1 2463322..2464392(-) (comGA) [Bacillus sp. MT(2024)]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=930043 VP472_RS11935 WP_070082113.1 2463322..2464392(-) (comGA) [Bacillus sp. MT(2024)]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACAGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCGGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374