Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACA343_RS28800 Genome accession   NZ_CP166821
Coordinates   6162685..6163308 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain J5083553     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6157685..6168308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA343_RS28780 (ACA343_28780) aroB 6158280..6159386 (-) 1107 WP_019484454.1 3-dehydroquinate synthase -
  ACA343_RS28785 (ACA343_28785) aroK 6159436..6159954 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACA343_RS28790 (ACA343_28790) pilQ 6159966..6162110 (-) 2145 WP_015503770.1 type 4a pilus secretin PilQ Machinery gene
  ACA343_RS28795 (ACA343_28795) comL/pilP 6162164..6162688 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACA343_RS28800 (ACA343_28800) pilO 6162685..6163308 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACA343_RS28805 (ACA343_28805) pilN 6163305..6163901 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACA343_RS28810 (ACA343_28810) pilM 6163901..6164965 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACA343_RS28815 (ACA343_28815) - 6165150..6167621 (+) 2472 WP_019484455.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=928892 ACA343_RS28800 WP_003103268.1 6162685..6163308(-) (pilO) [Pseudomonas aeruginosa strain J5083553]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=928892 ACA343_RS28800 WP_003103268.1 6162685..6163308(-) (pilO) [Pseudomonas aeruginosa strain J5083553]
ATGAGTCTGGCCAGTTCCCTGGAAAGTTTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401