Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SA496_RS23915 Genome accession   NZ_CP143312
Coordinates   5080168..5081232 (-) Length   354 a.a.
NCBI ID   WP_330114581.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5075168..5086232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS23890 (SA496_23890) aroK 5075720..5076238 (-) 519 WP_077524753.1 shikimate kinase AroK -
  SA496_RS23895 (SA496_23895) pilQ 5076244..5078346 (-) 2103 WP_120654170.1 type IV pilus secretin PilQ Machinery gene
  SA496_RS23900 (SA496_23900) pilP 5078404..5078937 (-) 534 WP_120654169.1 type 4a pilus biogenesis lipoprotein PilP -
  SA496_RS23905 (SA496_23905) pilO 5078934..5079557 (-) 624 WP_120654168.1 type 4a pilus biogenesis protein PilO -
  SA496_RS23910 (SA496_23910) pilN 5079554..5080168 (-) 615 WP_153918161.1 type 4a pilus biogenesis protein PilN -
  SA496_RS23915 (SA496_23915) pilM 5080168..5081232 (-) 1065 WP_330114581.1 pilus assembly protein PilM Machinery gene
  SA496_RS23920 (SA496_23920) - 5081417..5083852 (+) 2436 WP_162945865.1 penicillin-binding protein 1A -
  SA496_RS23925 (SA496_23925) - 5083909..5085177 (-) 1269 WP_120654164.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37879.13 Da        Isoelectric Point: 4.4486

>NTDB_id=928651 SA496_RS23915 WP_330114581.1 5080168..5081232(-) (pilM) [Pseudomonas sp. JS3066]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVVTKARTGVKVAAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLSAQLGDSHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=928651 SA496_RS23915 WP_330114581.1 5080168..5081232(-) (pilM) [Pseudomonas sp. JS3066]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCACTCCCTCCAAATGCGGTTGTCGAAAAGAACA
TCGCGGAACTGGAGGGCGTTGGGCAGGCGCTTTCCCGCGTTGTCACCAAGGCGCGAACCGGGGTCAAGGTCGCTGCGGTC
GCCGTGGCAGGCTCGGCCGTGATTACCAAGACCATCGAGATGGAAGCCGGTCTCTCCGATGATGATCTGGAGAATCAGCT
GAAAATCGAGGCCGACCAATACATTCCCTATCCGTTGGAAGAAGTCGCCATCGACTTCGAGGTGCAAGGCGCCTCGGCTC
GCAATCCGGAGCGGGTCGACGTACTTCTGGCTGCTTGCCGGAAGGAAAACGTCGAAGTACGTGAGGCAGCGCTTGCCCTG
GCAGGACTTACGGCCAAGGTCGTCGATGTCGAGGCTTACGCGTTGGAACGCGCCTATTCACTGCTTTCGGCCCAGCTCGG
TGATTCCCACGACGAACTTACCGTGGCGGTTGTTGACATCGGCGCCACCATGACGACCCTCAGTGTGCTGCACAACGGTC
GTACCATTTATACGCGGGAGCAGCTTTTCGGTGGTCGCCAGTTGACCGAGGAGATTCAGCGTCGCTATGGGCTTTCGGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGCGGTCTTCCGGATGACTACGACAGCGAGGTCCTTCAACCGTTCAAGGATGC
CGTCGTCCAGCAGGTATCCCGATCGCTGCAGTTCTTCTTTGCCGCGGGCCAGTACAACGACGTGGACTACATCCTCCTGG
CCGGCGGTACGGCCTCGATTCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGTACGCCGACCCTGGTTGCCAACCCC
TTCGCCGACATGGCGCTGAGTAGCAAGGTCAATGCGGGTGCCCTGGCCAGCGATGCGCCTGCGCTGATGATTGCTTGCGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469