Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SA496_RS14070 Genome accession   NZ_CP143312
Coordinates   3032395..3033600 (-) Length   401 a.a.
NCBI ID   WP_178123535.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3027395..3038600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS14050 (SA496_14050) - 3027629..3028327 (-) 699 WP_120655174.1 CoA transferase subunit A -
  SA496_RS14055 (SA496_14055) - 3028453..3029361 (+) 909 WP_120650550.1 LysR family transcriptional regulator -
  SA496_RS14060 (SA496_14060) - 3029421..3031376 (+) 1956 WP_120650549.1 acetoacetate--CoA ligase -
  SA496_RS14065 (SA496_14065) - 3031500..3032096 (-) 597 WP_330115595.1 DJ-1/PfpI family protein -
  SA496_RS14070 (SA496_14070) pilU 3032395..3033600 (-) 1206 WP_178123535.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SA496_RS14075 (SA496_14075) - 3033719..3033997 (+) 279 WP_028627186.1 peptidylprolyl isomerase -
  SA496_RS14080 (SA496_14080) - 3034075..3034374 (-) 300 WP_120650545.1 hypothetical protein -
  SA496_RS14085 (SA496_14085) - 3034461..3034646 (-) 186 WP_120650543.1 hypothetical protein -
  SA496_RS14090 (SA496_14090) - 3035136..3035492 (-) 357 WP_330115596.1 restriction endonuclease -
  SA496_RS14095 (SA496_14095) - 3035630..3036583 (-) 954 WP_330115597.1 hypothetical protein -
  SA496_RS14100 (SA496_14100) - 3036824..3037009 (-) 186 WP_330115598.1 hypothetical protein -
  SA496_RS14105 (SA496_14105) - 3037384..3037590 (-) 207 WP_330115599.1 hypothetical protein -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 44252.58 Da        Isoelectric Point: 6.1307

>NTDB_id=928643 SA496_RS14070 WP_178123535.1 3032395..3033600(-) (pilU) [Pseudomonas sp. JS3066]
MDLSAMLKILASQDGSDLYLSTGAPPCAKFNGLLRPLSNEPLKAGDVARIAESIMDPEQRAEFDRELEMNLAISLPNIGR
FRINIFRQRNEVSIVARNIKLDIPRFEDLKLPEVLLKMVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNTGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTSTISDLIKRGEFHSIKEIMEKSRGLGMQTFDQAL
FDLVVEGAIPEDEAIKNADSANNLRLKLKLYRDGPAAAPAPTAPAPAPVAAPSATQDAASWGLELKLEDLEEDGPEEPAP
R

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=928643 SA496_RS14070 WP_178123535.1 3032395..3033600(-) (pilU) [Pseudomonas sp. JS3066]
ATGGACCTCTCCGCGATGCTGAAAATCCTGGCCAGCCAGGATGGTTCCGACCTCTACCTGTCCACGGGCGCGCCGCCCTG
TGCCAAGTTCAACGGCCTGTTGCGGCCGCTCAGCAATGAGCCCCTGAAGGCCGGTGACGTGGCCAGGATCGCCGAATCGA
TCATGGACCCCGAGCAACGGGCCGAGTTCGACCGCGAACTGGAGATGAACCTGGCCATCTCGCTGCCGAACATCGGCCGC
TTCCGGATCAATATCTTCCGCCAGCGCAACGAGGTATCCATCGTCGCGCGGAACATCAAGCTGGATATCCCGCGCTTCGA
AGACCTCAAGCTGCCCGAAGTGCTGCTCAAGATGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGCACTGGCT
CCGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACACCGGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAATACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACACTGCGCCAGGCGCCGGACGTGATTCTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCGCCGATACCGGTCACCTTGCGATCTCCACGCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGAACGTCGCCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCTTTCGTTTCCCAGCG
CCTGGTGCGCACACAGGACGGCAAGCGCCGCGCGGCGGTGGAAGTGTTACTGGGTACCTCCACCATCAGCGACCTCATCA
AGCGCGGCGAATTCCACTCCATCAAGGAGATCATGGAGAAGTCCCGTGGCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTAGTGGAGGGCGCCATTCCCGAAGACGAAGCGATCAAGAACGCCGATTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTCTATCGCGATGGTCCTGCTGCAGCCCCAGCACCGACAGCCCCCGCCCCGGCGCCAGTTGCTGCTCCGAGCG
CCACCCAGGATGCCGCCAGCTGGGGGCTGGAGCTGAAGCTGGAAGACCTGGAGGAGGATGGGCCGGAGGAGCCAGCGCCT
CGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.41

87.531

0.494
  pilU Acinetobacter baylyi ADP1

53.409

87.781

0.469
  pilU Vibrio cholerae strain A1552

52.975

88.03

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.535

96.509

0.382