Detailed information NTG0927513

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VP464_RS04840
• Genome accession: NZ_CP142892
• Coordinates: 999864..1000922 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain B20AB01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 994864..1005922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP464_RS04825 (VP464_04830)	-	995969..996301 (+)	333	WP_000169594.1	hypothetical protein	-
VP464_RS04830 (VP464_04835)	-	996304..997128 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
VP464_RS04835 (VP464_04840)	ponA	997146..999701 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
VP464_RS04840 (VP464_04845)	pilM	999864..1000922 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
VP464_RS04845 (VP464_04850)	pilN	1000922..1001563 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
VP464_RS04850 (VP464_04855)	pilO	1001560..1002300 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
VP464_RS04855 (VP464_04860)	pilP	1002311..1002838 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
VP464_RS04860 (VP464_04865)	pilQ	1002906..1005068 (+)	2163	WP_033503048.1	type IV pilus secretin PilQ family protein	Machinery gene
VP464_RS04865 (VP464_04870)	aroK	1005080..1005649 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=927513 VP464_RS04840 WP_002014066.1 999864..1000922(+) (pilM) [Acinetobacter baumannii strain B20AB01]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=927513 VP464_RS04840 WP_002014066.1 999864..1000922(+) (pilM) [Acinetobacter baumannii strain B20AB01]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423