Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCU90_RS16040 Genome accession   NZ_AP025508
Coordinates   3564382..3565011 (-) Length   209 a.a.
NCBI ID   WP_061023572.1    Uniprot ID   -
Organism   Vibrio splendidus strain LMG 19031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3559382..3570011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS16020 aroB 3560314..3561402 (-) 1089 WP_004729663.1 3-dehydroquinate synthase -
  OCU90_RS16025 aroK 3561428..3561946 (-) 519 WP_004729664.1 shikimate kinase AroK -
  OCU90_RS16030 pilQ 3562123..3563841 (-) 1719 WP_061023567.1 type IV pilus secretin PilQ Machinery gene
  OCU90_RS16035 pilP 3563877..3564398 (-) 522 WP_372320604.1 pilus assembly protein PilP Machinery gene
  OCU90_RS16040 pilO 3564382..3565011 (-) 630 WP_061023572.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU90_RS16045 pilN 3565004..3565618 (-) 615 WP_061023574.1 PilN domain-containing protein Machinery gene
  OCU90_RS16050 pilM 3565602..3566627 (-) 1026 WP_061023576.1 type IV pilus assembly protein PilM Machinery gene
  OCU90_RS16055 - 3566779..3569337 (+) 2559 WP_061023578.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24322.91 Da        Isoelectric Point: 4.7890

>NTDB_id=92327 OCU90_RS16040 WP_061023572.1 3564382..3565011(-) (pilO) [Vibrio splendidus strain LMG 19031]
MANLQNKISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKRQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=92327 OCU90_RS16040 WP_061023572.1 3564382..3565011(-) (pilO) [Vibrio splendidus strain LMG 19031]
ATGGCTAACCTTCAAAATAAGATTAGTTGGCAAAACAGGATGAGCTTGCAAGATCTCGATGTTGATGAGATCACCGAATG
GCCATTGTTGCCTCAGCTTCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGC
CTTTGGATGATGAGCTACAGCAGATGAAGCGACAAGAGCAGACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCGGCCTTACCTAAGCTTCAGAGCCAGCTGGATGAGCTAACTAGCCGTTATGACTATCTATTGGAGCAATTGCCCGTTCA
AAAAGAGCTCGCCAGTATGTTGGCCTCTGTGAACGAGCTTGGATTGGATAATAAGCTGACATTTACGCGAATTGACTGGG
GGCAGAAGCAGAACAAAGAGTTTCTTTATCGTTTACCGCTCAACATCGAGCTGACGGGTGACTACCACGAGATTGGTGAT
TTCTCTGCGGCTATTGCTAAGCTGCCGCGCATCATCAGCTTTGATGACGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCATTTCAGGGTTCGAGCTTATACCTACCAATTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.648

92.344

0.699
  pilO Vibrio campbellii strain DS40M4

73.514

88.517

0.651

Multiple sequence alignment