Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OCU90_RS16030 Genome accession   NZ_AP025508
Coordinates   3562123..3563841 (-) Length   572 a.a.
NCBI ID   WP_061023567.1    Uniprot ID   -
Organism   Vibrio splendidus strain LMG 19031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3557123..3568841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS16010 - 3557799..3558647 (-) 849 WP_004729661.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  OCU90_RS26180 - 3558717..3559061 (-) 345 Protein_3086 SPOR domain-containing protein -
  OCU90_RS16015 - 3559127..3560234 (-) 1108 Protein_3087 ATP-binding protein -
  OCU90_RS16020 aroB 3560314..3561402 (-) 1089 WP_004729663.1 3-dehydroquinate synthase -
  OCU90_RS16025 aroK 3561428..3561946 (-) 519 WP_004729664.1 shikimate kinase AroK -
  OCU90_RS16030 pilQ 3562123..3563841 (-) 1719 WP_061023567.1 type IV pilus secretin PilQ Machinery gene
  OCU90_RS16035 pilP 3563877..3564398 (-) 522 WP_372320604.1 pilus assembly protein PilP Machinery gene
  OCU90_RS16040 pilO 3564382..3565011 (-) 630 WP_061023572.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU90_RS16045 pilN 3565004..3565618 (-) 615 WP_061023574.1 PilN domain-containing protein Machinery gene
  OCU90_RS16050 pilM 3565602..3566627 (-) 1026 WP_061023576.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62012.79 Da        Isoelectric Point: 4.4494

>NTDB_id=92325 OCU90_RS16030 WP_061023567.1 3562123..3563841(-) (pilQ) [Vibrio splendidus strain LMG 19031]
MIKGISGLVSKALQGFAVSVMLVFSVLGHAESLPNQLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELLNTQ
VNDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATIAGDYQYEYNLKGRFIEVVISKPVINEATKEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFKVGGSIEGNHPSQITPYDDNGGNSAIDDFLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=92325 OCU90_RS16030 WP_061023567.1 3562123..3563841(-) (pilQ) [Vibrio splendidus strain LMG 19031]
ATGATTAAAGGAATAAGTGGATTAGTGAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTAGTCTTTAGTGTGCT
CGGTCACGCCGAGAGCTTACCTAATCAATTGGAGAATATTGATTTTAGGGTAAACAAAGATAAAGACGCCGTCATTATTA
TTGAGCTGGCAACCAGCACCGCTGTGGTGGATGTTCAACGTGCTCAGGAAGGGTTAAGTATCGAATTACTCAATACTCAA
GTTAATGACGATAAGCTTTATCTTTTGGATGTGAAGGATTTCGCGACCCTAGTGGAAGGTATTGAAGTCTATAAAGAGAC
GCCCAGCACACGACTGTTGGCGACGATCGCTGGTGACTATCAGTATGAATATAACCTAAAAGGTCGTTTCATAGAGGTGG
TGATCAGCAAGCCTGTTATCAATGAAGCGACCAAAGAGAAAAGCGTTTTAGAAAAAGAGGGCAAACTGATATCGATTAAC
TTCCAAGATATCCCGGTACGAAATGTCCTGCAGCTGATTGCTGACTACAACGATTTCAATCTCGTCGTGTCTGACTCGGT
AGCTGGTAACCTAACTTTACGTTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGACTAG
ATAAGCGCGTTGATGGTAATGTTATTTTGGTGGCGCCAAAAGCAGAACTCGATCTTCGAGAGCAACAAGCGTTAGAGAAA
TCTCGCTTGGAAGAGGAGTTAGGGGAGCTTAAGTCGGAAATCATCAAGATTAATTTTGCTAAAGCCACCGACATTGCCGA
CATGATTGGCGGTGAAGGTGCGGTGAGCATGTTGTCCGATCGTGGTTCGATCACCATTGATGAACGCACCAATTCCCTAT
TGATTCGTGAACTGGAAGAAAACATTGCCGTGATACGAGGCATCATTGAATCTTTGGACATCCCCGTGAAGCAAGTCCAG
ATAGAGGCGCGCATTGTAACCGTTACCGAGGGTAACCTTGATGAGTTAGGGGTGCGTTGGGGTGTTTCTTCCACCAACGG
TAGCTTCAAAGTTGGCGGCTCGATAGAGGGCAACCACCCATCGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTG
CGATTGACGATTTCCTCAACGTTAATCTAGGGGCGACATCTCCTAATGCATCAAGCATTGCGTTTCAGGTGGCCAAACTG
GGTTCAGATACCTTGCTTGATCTTGAATTGTCGGCACTACAACAAGAGTCAAAAGCTGAGATTATTTCCAGCCCACGTTT
AATCACTACCAATAAAAAGCCCGCTTATATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCGTCTTCAAGTGGTGCTA
CTTCGGTCGCATTTAAGAAAGCGGTGTTGAGTCTTAAGGTGACACCACAAATCACGCCCGACAATCGTTTGGTATTGGAT
TTAAGCGTGACTCAAGATAGGCCTGGGCAAGTAGTGAAAACCGGAACGGGTGAGGCTGTTGCCATTGATACTCAACGTAT
AGGGACGCAAGTGCTTGTTAATAATGGTGAAACAGTCGTGCTTGGCGGGATATTTCAGCACAGTGTGAGCAGTACAGTGG
ACAAAGTTCCTCTGTTAGGAGACCTGCCAGTTTTGGGTGCATTATTCCGTCGTAGCTATGAAAATGTGGGTAAAAGTGAA
CTACTTATTTTTGTTACACCCAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.875

100

0.781
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.875

100

0.781
  pilQ Vibrio campbellii strain DS40M4

80.882

95.105

0.769
  pilQ Legionella pneumophila strain ERS1305867

43.04

95.455

0.411
  pilQ Legionella pneumophila strain Lp02

43.04

95.455

0.411
  pilQ Pseudomonas aeruginosa PAK

41.145

97.727

0.402
  comQ Acinetobacter baylyi ADP1

39.399

98.951

0.39
  pilQ Acinetobacter baumannii strain A118

37.824

100

0.383
  pilQ Acinetobacter baumannii D1279779

37.824

100

0.383

Multiple sequence alignment