Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCU90_RS16050 Genome accession   NZ_AP025508
Coordinates   3565602..3566627 (-) Length   341 a.a.
NCBI ID   WP_061023576.1    Uniprot ID   -
Organism   Vibrio splendidus strain LMG 19031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3560602..3571627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS16025 aroK 3561428..3561946 (-) 519 WP_004729664.1 shikimate kinase AroK -
  OCU90_RS16030 pilQ 3562123..3563841 (-) 1719 WP_061023567.1 type IV pilus secretin PilQ Machinery gene
  OCU90_RS16035 pilP 3563877..3564398 (-) 522 WP_372320604.1 pilus assembly protein PilP Machinery gene
  OCU90_RS16040 pilO 3564382..3565011 (-) 630 WP_061023572.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU90_RS16045 pilN 3565004..3565618 (-) 615 WP_061023574.1 PilN domain-containing protein Machinery gene
  OCU90_RS16050 pilM 3565602..3566627 (-) 1026 WP_061023576.1 type IV pilus assembly protein PilM Machinery gene
  OCU90_RS16055 - 3566779..3569337 (+) 2559 WP_061023578.1 penicillin-binding protein 1A -
  OCU90_RS16060 oxyR 3569450..3570343 (-) 894 WP_017080198.1 DNA-binding transcriptional regulator OxyR -
  OCU90_RS16065 - 3570342..3570521 (+) 180 WP_017077611.1 hypothetical protein -
  OCU90_RS16070 - 3570565..3571293 (+) 729 WP_017073712.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38131.66 Da        Isoelectric Point: 6.9699

>NTDB_id=92329 OCU90_RS16050 WP_061023576.1 3565602..3566627(-) (pilM) [Vibrio splendidus strain LMG 19031]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQMASRLYPEKKNWLLVDVGIDQTSLGIIPQGLAPFYKDIAFGTKDLRSNGPASDIESVFATTEDTH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPFSLFENKVAKRRRLPTNWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92329 OCU90_RS16050 WP_061023576.1 3565602..3566627(-) (pilM) [Vibrio splendidus strain LMG 19031]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCTGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGGCTGCCTTTTGGCTGTTGCAACGTCGCCATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAGGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCGATAGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAAGTATACGCCACTCGTAAAGAAGTGGTGGATAGCCGAGCGGATGCTTTGACCAAAGCTGGTTTTAAACCT
GTGGTGGTAGACACGCAGGCGCATGGGTTGCTTAACATCTGGCAAATGGCATCGCGCCTGTACCCCGAAAAGAAAAATTG
GCTGCTGGTGGATGTGGGAATCGACCAAACGTCGTTAGGCATTATTCCACAAGGTTTGGCACCGTTTTATAAAGATATCG
CTTTTGGTACCAAAGATCTTCGCAGCAATGGTCCTGCAAGTGATATCGAGAGTGTGTTCGCCACCACTGAAGATACACAT
CAATTTATTGTTAATTTGATTGAGAAGCTCAAGCGTCAATTACAGCTCTATTCATCAGGAAATGCACAGCAACCTATCTC
AGGGATCTGGCTGATGGGTGAGGGCGCCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCATTTTCAGTTAAGTTGCG
AGTCACTGAACCCTTTCTCTCTATTTGAGAATAAGGTGGCGAAGCGACGTCGGTTACCAACGAACTGGCAACATTTCGGT
ATTGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.412

99.707

0.543
  pilM Vibrio campbellii strain DS40M4

47.674

100

0.481

Multiple sequence alignment