Detailed information NTG0921858

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VQ574_RS19160
• Genome accession: NZ_CP142122
• Coordinates: 4092107..4093252 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain XN05-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4087107..4098252

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VQ574_RS19135 (VQ574_19135)	-	4087190..4088206 (+)	1017	WP_063540691.1	aspartate carbamoyltransferase catalytic subunit	-
VQ574_RS19140 (VQ574_19140)	-	4088206..4089480 (+)	1275	WP_110778830.1	dihydroorotase	-
VQ574_RS19145 (VQ574_19145)	-	4089535..4089930 (-)	396	WP_017245432.1	NINE protein	-
VQ574_RS19150 (VQ574_19150)	-	4090062..4091276 (+)	1215	WP_267931429.1	putative DNA modification/repair radical SAM protein	-
VQ574_RS19155 (VQ574_19155)	-	4091276..4092100 (+)	825	WP_326429072.1	TIGR03915 family putative DNA repair protein	-
VQ574_RS19160 (VQ574_19160)	pilU	4092107..4093252 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VQ574_RS19165 (VQ574_19165)	pilT	4093313..4094347 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
VQ574_RS19170 (VQ574_19170)	-	4094414..4095103 (+)	690	WP_230724212.1	YggS family pyridoxal phosphate-dependent enzyme	-
VQ574_RS19175 (VQ574_19175)	proC	4095114..4095938 (+)	825	WP_200597334.1	pyrroline-5-carboxylate reductase	-
VQ574_RS19180 (VQ574_19180)	-	4095948..4096538 (+)	591	WP_326429073.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=921858 VQ574_RS19160 WP_026006585.1 4092107..4093252(-) (pilU) [Stutzerimonas frequens strain XN05-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=921858 VQ574_RS19160 WP_026006585.1 4092107..4093252(-) (pilU) [Stutzerimonas frequens strain XN05-1]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAAAATCACGAATGCAACTTCGCCATCAGCGCCCGCGGTATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTCCTGAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCTAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAAACCGGACACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAACA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTATTGATCAACACCCCGCTGGCCGCGGACCTTA
TCCGCAAGGGCGAAGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCACCGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388