Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VOI46_RS02655 Genome accession   NZ_CP142030
Coordinates   611259..612392 (+) Length   377 a.a.
NCBI ID   WP_004586841.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. CuT4-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606259..617392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI46_RS02625 (VOI46_02625) - 606989..607588 (-) 600 WP_004586835.1 XTP/dITP diphosphatase -
  VOI46_RS02630 (VOI46_02630) - 607593..608036 (-) 444 WP_004586836.1 DUF4426 domain-containing protein -
  VOI46_RS02635 (VOI46_02635) - 608094..608627 (-) 534 WP_004586837.1 YggT family protein -
  VOI46_RS02640 (VOI46_02640) proC 608654..609475 (-) 822 WP_004586838.1 pyrroline-5-carboxylate reductase -
  VOI46_RS02645 (VOI46_02645) - 609493..610206 (-) 714 WP_004586839.1 YggS family pyridoxal phosphate-dependent enzyme -
  VOI46_RS02650 (VOI46_02650) - 610209..611249 (+) 1041 Protein_514 type IV pilus twitching motility protein PilT -
  VOI46_RS02655 (VOI46_02655) pilU 611259..612392 (+) 1134 WP_004586841.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VOI46_RS02660 (VOI46_02660) ruvX 612526..612975 (-) 450 WP_004586842.1 Holliday junction resolvase RuvX -
  VOI46_RS02665 (VOI46_02665) - 612985..613542 (-) 558 WP_004586843.1 YqgE/AlgH family protein -
  VOI46_RS02670 (VOI46_02670) gshB 613667..614617 (-) 951 WP_324738172.1 glutathione synthase -
  VOI46_RS02675 (VOI46_02675) rsmE 614693..615427 (-) 735 WP_004586845.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VOI46_RS02680 (VOI46_02680) - 615500..615976 (-) 477 WP_004586846.1 hypothetical protein -
  VOI46_RS02685 (VOI46_02685) - 616058..616951 (-) 894 WP_004586847.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41803.74 Da        Isoelectric Point: 6.3724

>NTDB_id=921183 VOI46_RS02655 WP_004586841.1 611259..612392(+) (pilU) [Pseudoalteromonas sp. CuT4-3]
MNLSLNHFLHIMIDKKGSDLFVSSQLPVSAKINGELIALSDDKLSDEQSLALVESAMSVKQKNEFHTTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDISDLGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEILLNSPMVAELIKNGDIGGIKEAMSKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKNSN

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=921183 VOI46_RS02655 WP_004586841.1 611259..612392(+) (pilU) [Pseudoalteromonas sp. CuT4-3]
ATGAATTTATCTTTAAATCACTTTTTACACATAATGATTGATAAAAAAGGCTCGGATTTATTTGTCTCTAGCCAATTACC
AGTCAGTGCAAAAATTAATGGTGAGCTTATCGCTCTTAGCGACGATAAGTTAAGTGATGAGCAATCGCTTGCGCTTGTAG
AGTCAGCGATGAGCGTTAAACAAAAAAACGAATTTCATACTACTAAAGAGTGTAACTTTGCGATAGCCACAGACGAAGGG
CGTTTTCGTATATCGGCTTTTTGGCAGCGCGATAGAGCGGGTATGGTTATTCGCCGCATTGTAACGCAAATACCTGATAT
AAGTGATTTAGGGCTGCCCTCTACACTTACCGATGTGATTATGTCAAAGCGCGGGTTAGTGCTGTTTGTTGGTGGTACTG
GCACGGGTAAATCAACTTCACTGGCTGCCTTAATCGGCTACAGAAACCGCAACCAGCGCGGCCATATACTCACCATTGAA
GATCCGATTGAGTTTGTGCACGAGCACCGTAAAAGTATTATTACTCAGCGCGAAGTAGGGCTTGATACCGACAGCTTTGA
GTCGGCACTTAAAAGCTCTTTACGCCAAGCTCCCGATGTTATTTTAATTGGTGAAATACGCTCGCAAGAAACCATGGAAT
ACGCACTGAGCTTTGCCGAAACGGGTCACTTATGTGTGGCTACATTGCATGCTAATAATGCCAACCAAGCCATTGACCGT
ATTATGCACTTAGTGCCTAAAGAAAAACACGACAAGCTTAAGTACGACTTAGCGCTAAATTTACGCGCCATTGTTGCCCA
GCAACTTATTCCCACTTCTGATGGGGAAGGGCGTGTAGCCGCGATTGAAATATTATTAAACTCACCCATGGTGGCAGAGC
TAATTAAAAATGGGGATATTGGTGGCATAAAAGAGGCCATGTCTAAGTCTAAAGAAATGGGTATGCAAACCTTTGACCAA
GCCTTATTTGAGCTATACAAACAGCAGCGTATTAATTACGCCGATGCACTACATCATGCTGATTCGCCAAACGATTTACG
CTTAATGATTAAGCTGCGCAATAATGAGCAACAAGGTGCAGGATTTTTACAAGGTGTAACTATTGATGGACTAGATGATA
AAAACAGCAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.067

99.469

0.568
  pilU Acinetobacter baylyi ADP1

56.233

100

0.562
  pilU Vibrio cholerae strain A1552

55.586

97.347

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.659

95.756

0.408