Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   VDS58_RS14255 Genome accession   NZ_CP141997
Coordinates   2614369..2614986 (-) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain PM0031     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609369..2619986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS58_RS14225 (VDS58_14225) rpsT 2609669..2609935 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  VDS58_RS14230 (VDS58_14230) holA 2609950..2610993 (-) 1044 WP_159376962.1 DNA polymerase III subunit delta -
  VDS58_RS14235 (VDS58_14235) - 2611033..2611146 (-) 114 WP_134975484.1 hypothetical protein -
  VDS58_RS14240 (VDS58_14240) yqzM 2611223..2611357 (+) 135 WP_003229983.1 YqzM family protein -
  VDS58_RS14245 (VDS58_14245) comEC 2611399..2613729 (-) 2331 WP_159376963.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  VDS58_RS14250 (VDS58_14250) comEB 2613733..2614302 (-) 570 WP_003229978.1 ComE operon protein 2 -
  VDS58_RS14255 (VDS58_14255) comEA 2614369..2614986 (-) 618 WP_032726193.1 competence protein ComEA Machinery gene
  VDS58_RS14260 (VDS58_14260) comER 2615070..2615891 (+) 822 WP_159376964.1 late competence protein ComER -
  VDS58_RS14265 (VDS58_14265) yqeM 2615957..2616700 (-) 744 WP_032726197.1 class I SAM-dependent DNA methyltransferase -
  VDS58_RS14270 (VDS58_14270) rsfS 2616697..2617053 (-) 357 WP_014480315.1 ribosome silencing factor -
  VDS58_RS14275 (VDS58_14275) yqeK 2617071..2617631 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  VDS58_RS14280 (VDS58_14280) nadD 2617621..2618190 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  VDS58_RS14285 (VDS58_14285) yhbY 2618202..2618492 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  VDS58_RS14290 (VDS58_14290) aroE 2618486..2619328 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=920737 VDS58_RS14255 WP_032726193.1 2614369..2614986(-) (comEA) [Bacillus subtilis strain PM0031]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=920737 VDS58_RS14255 WP_032726193.1 2614369..2614986(-) (comEA) [Bacillus subtilis strain PM0031]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376