Detailed information NTG0920560

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VK748_RS28150
• Genome accession: NZ_CP141948
• Coordinates: 6229550..6230752 (-)
• Length: 400 a.a.
• NCBI ID: WP_324709874.1
• Uniprot ID: -
• Organism: Pseudomonas citronellolis strain G5.41a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6224550..6235752

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VK748_RS28125 (VK748_28125)	-	6224930..6225874 (-)	945	WP_324709868.1	AraC family transcriptional regulator	-
VK748_RS28130 (VK748_28130)	-	6225968..6226801 (+)	834	WP_324709869.1	oxidoreductase	-
VK748_RS28135 (VK748_28135)	-	6227313..6227585 (-)	273	WP_009619536.1	hypothetical protein	-
VK748_RS28140 (VK748_28140)	-	6228048..6229004 (-)	957	WP_324709871.1	MBL fold metallo-hydrolase	-
VK748_RS28145 (VK748_28145)	-	6229211..6229354 (-)	144	WP_324709873.1	hypothetical protein	-
VK748_RS28150 (VK748_28150)	pilU	6229550..6230752 (-)	1203	WP_324709874.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VK748_RS28155 (VK748_28155)	-	6230944..6231222 (+)	279	WP_009619516.1	peptidylprolyl isomerase	-
VK748_RS28160 (VK748_28160)	-	6231236..6231598 (+)	363	WP_009619514.1	MmcQ/YjbR family DNA-binding protein	-
VK748_RS28165 (VK748_28165)	-	6231595..6232152 (+)	558	WP_064583536.1	putative glycolipid-binding domain-containing protein	-
VK748_RS28170 (VK748_28170)	-	6232422..6233621 (+)	1200	WP_324709876.1	MFS transporter	-
VK748_RS28175 (VK748_28175)	-	6234461..6235741 (+)	1281	WP_324709877.1	OprD family porin	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 43929.08 Da
• Isoelectric Point: 6.0779

>NTDB_id=920560 VK748_RS28150 WP_324709874.1 6229550..6230752(-) (pilU) [Pseudomonas citronellolis strain G5.41a]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPAAAQTQAASAQPATPPAPAVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide

• Length: 1203 bp

>NTDB_id=920560 VK748_RS28150 WP_324709874.1 6229550..6230752(-) (pilU) [Pseudomonas citronellolis strain G5.41a]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTTTACCTTTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAACCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAGCAGAAGGCCGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTATCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
AGACCTGAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATTACCATCGAGGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGTTCGATCAAGGAGATCATGGACAAGTCCCGTGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAACCCGGCCGCGGCCCAGACCCAGGCCGCCAGCGCCCAGCCGGCAACCCCGCCCGCGCCGG
CGGTGGAAGCGTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.695	87.75	0.497
pilU	Acinetobacter baylyi ADP1	53.409	88	0.47
pilU	Vibrio cholerae strain A1552	52.125	88.25	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.42	95.25	0.385