Detailed information NTG0920543

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VK748_RS07975
• Genome accession: NZ_CP141948
• Coordinates: 1666931..1668076 (-)
• Length: 381 a.a.
• NCBI ID: WP_009615238.1
• Uniprot ID: A0A1A5D0E6
• Organism: Pseudomonas citronellolis strain G5.41a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1661931..1673076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VK748_RS07960 (VK748_07960)	-	1663384..1664556 (-)	1173	WP_324710713.1	cystathionine gamma-synthase	-
VK748_RS07965 (VK748_07965)	-	1664553..1665926 (-)	1374	WP_058072678.1	pyridoxal-phosphate dependent enzyme	-
VK748_RS07970 (VK748_07970)	-	1666233..1666637 (-)	405	WP_043269499.1	NINE protein	-
VK748_RS07975 (VK748_07975)	pilU	1666931..1668076 (-)	1146	WP_009615238.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VK748_RS07980 (VK748_07980)	pilT	1668181..1669215 (-)	1035	WP_009615237.1	type IV pilus twitching motility protein PilT	Machinery gene
VK748_RS07985 (VK748_07985)	-	1669289..1669978 (+)	690	WP_324710714.1	YggS family pyridoxal phosphate-dependent enzyme	-
VK748_RS07990 (VK748_07990)	proC	1669995..1670813 (+)	819	WP_269961755.1	pyrroline-5-carboxylate reductase	-
VK748_RS07995 (VK748_07995)	-	1670824..1671417 (+)	594	WP_009615234.1	YggT family protein	-
VK748_RS08000 (VK748_08000)	-	1671417..1671713 (+)	297	WP_249918056.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42685.06 Da
• Isoelectric Point: 6.7792

>NTDB_id=920543 VK748_RS07975 WP_009615238.1 1666931..1668076(-) (pilU) [Pseudomonas citronellolis strain G5.41a]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSEDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=920543 VK748_RS07975 WP_009615238.1 1666931..1668076(-) (pilU) [Pseudomonas citronellolis strain G5.41a]
ATGGAATTCGAGAAGCTTCTGCGGCTGATGGTGGAGAAGGGCGCCTCGGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCGCCCGAGCAGACCCGCGAGACCGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGTTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACACCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAATACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGTGAGGTGCATGAGCTCAAGCCGTTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACTCCCAGGGCGAGATCACCTATGAGGATGCGCTGGCCTATGCCGACTCGGCCAACGATCTGCGGTT
GATGATCAAGCTTGGGTCGGAGACCGATGCGGATCATCTGTCGAGCATGACTCAGGGGTTGACCCTGGAGCTGAGCGAAG
ACGATCCGAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

3D structure

• AlphaFold DB: A0A1A5D0E6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.664	100	0.877
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386