NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	VO179_RS05250	Genome accession	NZ_CP141827
Coordinates	1197340..1198410 (-)	Length	356 a.a.
NCBI ID	WP_007408320.1	Uniprot ID	-
Organism	Bacillus velezensis strain XHA14
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1192340..1203410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VO179_RS05200 (VO179_05200)	sipW	1192400..1192984 (-)	585	WP_015240205.1	signal peptidase I SipW	-
VO179_RS05205 (VO179_05205)	tapA	1192956..1193627 (-)	672	WP_324636720.1	amyloid fiber anchoring/assembly protein TapA	-
VO179_RS05210 (VO179_05210)	-	1193886..1194215 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
VO179_RS05215 (VO179_05215)	-	1194255..1194434 (-)	180	WP_003153093.1	YqzE family protein	-
VO179_RS05220 (VO179_05220)	comGG	1194491..1194868 (-)	378	WP_015417814.1	competence type IV pilus minor pilin ComGG	Machinery gene
VO179_RS05225 (VO179_05225)	comGF	1194869..1195264 (-)	396	WP_324636718.1	competence type IV pilus minor pilin ComGF	-
VO179_RS05230 (VO179_05230)	comGE	1195278..1195592 (-)	315	WP_324636717.1	competence type IV pilus minor pilin ComGE	-
VO179_RS05235 (VO179_05235)	comGD	1195576..1196013 (-)	438	WP_015417817.1	competence type IV pilus minor pilin ComGD	Machinery gene
VO179_RS05240 (VO179_05240)	comGC	1196003..1196269 (-)	267	WP_050515801.1	competence type IV pilus major pilin ComGC	Machinery gene
VO179_RS05245 (VO179_05245)	comGB	1196316..1197353 (-)	1038	WP_154817564.1	competence type IV pilus assembly protein ComGB	Machinery gene
VO179_RS05250 (VO179_05250)	comGA	1197340..1198410 (-)	1071	WP_007408320.1	competence type IV pilus ATPase ComGA	Machinery gene
VO179_RS05255 (VO179_05255)	-	1198603..1199553 (-)	951	WP_007408319.1	magnesium transporter CorA family protein	-
VO179_RS05260 (VO179_05260)	-	1199699..1201000 (+)	1302	WP_324636716.1	hemolysin family protein	-
VO179_RS05265 (VO179_05265)	-	1201038..1201868 (-)	831	WP_015417821.1	STAS domain-containing protein	-
VO179_RS05275 (VO179_05275)	-	1202075..1202455 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
VO179_RS05280 (VO179_05280)	-	1202692..1202937 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40530.79 Da Isoelectric Point: 9.1996

                                >NTDB_id=919875 VO179_RS05250 WP_007408320.1 1197340..1198410(-) (comGA) [Bacillus velezensis strain XHA14]

MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=919875 VO179_RS05250 WP_007408320.1 1197340..1198410(-) (comGA) [Bacillus velezensis strain XHA14]

GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGTTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0919875