Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCV36_RS01220 Genome accession   NZ_AP025483
Coordinates   273894..274493 (+) Length   199 a.a.
NCBI ID   WP_135457719.1    Uniprot ID   -
Organism   Vibrio echinoideorum strain DSM 107264     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268894..279493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV36_RS01200 - 268982..269161 (-) 180 WP_017073711.1 hypothetical protein -
  OCV36_RS01205 oxyR 269160..270053 (+) 894 WP_017073710.1 DNA-binding transcriptional regulator OxyR -
  OCV36_RS01210 - 270172..272733 (-) 2562 WP_167853048.1 penicillin-binding protein 1A -
  OCV36_RS01215 pilM 272885..273910 (+) 1026 WP_029224878.1 type IV pilus assembly protein PilM Machinery gene
  OCV36_RS01220 pilN 273894..274493 (+) 600 WP_135457719.1 PilN domain-containing protein Machinery gene
  OCV36_RS01225 pilO 274486..275115 (+) 630 WP_135457721.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV36_RS01230 pilP 275102..275620 (+) 519 WP_029224876.1 pilus assembly protein PilP Machinery gene
  OCV36_RS01235 pilQ 275656..277371 (+) 1716 WP_135457723.1 type IV pilus secretin PilQ Machinery gene
  OCV36_RS01240 aroK 277548..278066 (+) 519 WP_017073703.1 shikimate kinase AroK -
  OCV36_RS01245 aroB 278091..279179 (+) 1089 WP_017073702.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22888.36 Da        Isoelectric Point: 9.8373

>NTDB_id=91948 OCV36_RS01220 WP_135457719.1 273894..274493(+) (pilN) [Vibrio echinoideorum strain DSM 107264]
MLHQINLLPWRDEIRGQHKKRFVHLVILGTIIAFLGQWAMGNYFHGQHAKQQSRLSYLDKYIAELDQKIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLESTTASRKEKGAHHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=91948 OCV36_RS01220 WP_135457719.1 273894..274493(+) (pilN) [Vibrio echinoideorum strain DSM 107264]
ATGTTGCATCAAATTAACCTGCTGCCTTGGCGTGACGAGATTCGAGGTCAGCATAAAAAGCGTTTTGTTCATTTGGTTAT
TCTCGGCACCATTATAGCGTTCTTGGGGCAATGGGCGATGGGAAACTATTTTCATGGCCAACACGCCAAGCAGCAATCTC
GCCTCAGTTACCTCGATAAGTACATCGCAGAGCTCGACCAGAAGATCCAATCATTAAAAGTGGCGGAACAAGAACACAAA
GCCATTCTTACCAGACTCGATGTTGTGGAGTCATTACAACTCGGTCGCAATAAAACTACCGACTTTATGAACCTGATGCC
TGAGTTGATTCCCGAGGGTGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATTAGCGACA
GTACTGCTCGTCTCGCTACGATGTTGGACAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTCGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTTAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTGTCTTTAGAAAGCAC
AACCGCGAGCAGAAAAGAGAAGGGGGCGCATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

62.5

100

0.628
  pilN Vibrio campbellii strain DS40M4

61.828

93.467

0.578

Multiple sequence alignment