Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV36_RS01215 Genome accession   NZ_AP025483
Coordinates   272885..273910 (+) Length   341 a.a.
NCBI ID   WP_029224878.1    Uniprot ID   -
Organism   Vibrio echinoideorum strain DSM 107264     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267885..278910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV36_RS01195 - 268209..268937 (-) 729 WP_017073712.1 glutathione peroxidase -
  OCV36_RS01200 - 268982..269161 (-) 180 WP_017073711.1 hypothetical protein -
  OCV36_RS01205 oxyR 269160..270053 (+) 894 WP_017073710.1 DNA-binding transcriptional regulator OxyR -
  OCV36_RS01210 - 270172..272733 (-) 2562 WP_167853048.1 penicillin-binding protein 1A -
  OCV36_RS01215 pilM 272885..273910 (+) 1026 WP_029224878.1 type IV pilus assembly protein PilM Machinery gene
  OCV36_RS01220 pilN 273894..274493 (+) 600 WP_135457719.1 PilN domain-containing protein Machinery gene
  OCV36_RS01225 pilO 274486..275115 (+) 630 WP_135457721.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV36_RS01230 pilP 275102..275620 (+) 519 WP_029224876.1 pilus assembly protein PilP Machinery gene
  OCV36_RS01235 pilQ 275656..277371 (+) 1716 WP_135457723.1 type IV pilus secretin PilQ Machinery gene
  OCV36_RS01240 aroK 277548..278066 (+) 519 WP_017073703.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38220.80 Da        Isoelectric Point: 6.9830

>NTDB_id=91947 OCV36_RS01215 WP_029224878.1 272885..273910(+) (pilM) [Vibrio echinoideorum strain DSM 107264]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVSISVPENT
VISKVLQIESDLEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
ILVDTQAHGLLNIWQLASRLYPEKKNWLLVDVGIDQTSLGIIPQGSVPFYKDLAFGTQDLRGTDKPSDIEGGFVTAEETH
RFIVNLIEKLKRQLQLYSSVNTLQPIAGIWLMGEGASTPMLIDELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91947 OCV36_RS01215 WP_029224878.1 272885..273910(+) (pilM) [Vibrio echinoideorum strain DSM 107264]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCTGTGGTCCTAAAACCCGTTGGGGAGTC
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTCTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGTCGCAACGTCTCTATTTCGGTACCGGAGAACACA
GTGATCAGCAAAGTACTGCAAATAGAGAGTGATCTAGAAGACAGAGAAAAAGAGTTCTCGATCTATCAAACCTTTGCTCA
CCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTACCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCCGATGCGTTAACGAAAGCAGGGTTTAAACCT
ATCTTGGTTGATACACAGGCACATGGCCTTCTCAATATTTGGCAACTGGCTTCGCGTTTGTATCCAGAAAAAAAGAATTG
GTTGCTCGTGGATGTTGGCATCGACCAAACGTCGTTAGGGATTATCCCACAAGGTTCAGTACCTTTTTATAAAGACCTTG
CCTTTGGTACCCAAGATCTTCGCGGTACGGATAAACCTAGTGATATCGAAGGTGGGTTCGTCACAGCCGAGGAGACGCAC
CGATTTATTGTCAATTTAATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCGTCGGTGAATACGCTTCAACCTATTGC
AGGGATATGGCTGATGGGTGAGGGTGCAAGCACACCGATGCTCATTGATGAGCTAGAGCGACATTTTCAGTTGAGTTGTG
AGTCATTGAATCCTTTGTCTTTGTTTGAAAATAAGGTTGCGAAGAAGCGTCGACTACCTATGGATTGGCAACATTTTGGT
ATTGCGGCGGGTATGGCAATGAGTGGGCTTAAATGGCAAGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.867

99.413

0.545
  pilM Vibrio campbellii strain DS40M4

48.688

100

0.49

Multiple sequence alignment