Detailed information NTG0919178

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VLX10_RS02615
• Genome accession: NZ_CP141753
• Coordinates: 571134..572282 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2023CK-01336
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 566134..577282

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VLX10_RS02590 (VLX10_02590)	-	567285..567581 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
VLX10_RS02595 (VLX10_02595)	-	567578..568171 (-)	594	WP_003084544.1	YggT family protein	-
VLX10_RS02600 (VLX10_02600)	proC	568182..569003 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
VLX10_RS02605 (VLX10_02605)	-	569015..569707 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
VLX10_RS02610 (VLX10_02610)	pilT	569922..570956 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
VLX10_RS02615 (VLX10_02615)	pilU	571134..572282 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
VLX10_RS02620 (VLX10_02620)	czcD	572290..573189 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
VLX10_RS02625 (VLX10_02625)	-	573344..573748 (+)	405	WP_003084560.1	NINE protein	-
VLX10_RS02630 (VLX10_02630)	-	573991..575364 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
VLX10_RS02635 (VLX10_02635)	-	575361..576545 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=919178 VLX10_RS02615 WP_003110016.1 571134..572282(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01336]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=919178 VLX10_RS02615 WP_003110016.1 571134..572282(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01336]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382