Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   VNN53_RS01475 Genome accession   NZ_CP141720
Coordinates   297418..299628 (-) Length   736 a.a.
NCBI ID   WP_338962132.1    Uniprot ID   -
Organism   Lactococcus garvieae strain CAHFS22OV20.5B5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292418..304628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN53_RS01445 (VNN53_01445) atpA 292672..294180 (-) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -
  VNN53_RS01450 (VNN53_01450) - 294246..294773 (-) 528 WP_014024339.1 F0F1 ATP synthase subunit delta -
  VNN53_RS01455 (VNN53_01455) atpF 294788..295294 (-) 507 WP_004259173.1 F0F1 ATP synthase subunit B -
  VNN53_RS01460 (VNN53_01460) atpB 295308..296021 (-) 714 WP_004259176.1 F0F1 ATP synthase subunit A -
  VNN53_RS01465 (VNN53_01465) - 296076..296291 (-) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  VNN53_RS01470 (VNN53_01470) - 296487..297263 (-) 777 WP_074750667.1 alpha/beta hydrolase family protein -
  VNN53_RS01475 (VNN53_01475) comEC 297418..299628 (-) 2211 WP_338962132.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  VNN53_RS01480 (VNN53_01480) - 299648..300298 (-) 651 WP_338962135.1 helix-hairpin-helix domain-containing protein -
  VNN53_RS01485 (VNN53_01485) - 300356..301531 (-) 1176 WP_004259189.1 SLC13 family permease -
  VNN53_RS01490 (VNN53_01490) - 301697..303253 (-) 1557 WP_176490086.1 ABC-F family ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 85181.96 Da        Isoelectric Point: 9.7959

>NTDB_id=918834 VNN53_RS01475 WP_338962132.1 297418..299628(-) (comEC) [Lactococcus garvieae strain CAHFS22OV20.5B5]
MKQKFSLIYFVYLLVFVYFLIFSFSWPLLILSLFSLFIAFYRQYYSLLVLLLCFSLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYLEGTLEIPEEQRNFSGFDYRKYLRTQGIHRL
LKIEKIHGFEEKQDFDLRLVRRKAILWTQKHFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTPGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVLESIVRWVGDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIVLFLLFFFVKNPKHPSITMVD
VGQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDILIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRFQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKIALISAGKNNRYKHPNQETLQQFAARGIKTYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=918834 VNN53_RS01475 WP_338962132.1 297418..299628(-) (comEC) [Lactococcus garvieae strain CAHFS22OV20.5B5]
ATGAAACAAAAATTTTCGCTTATCTATTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTCTCTTCATCGCTTTTTATCGTCAATACTATAGTTTGTTAGTTCTTTTACTGTGCT
TTAGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTTCAAGCTTT
TTACACCCTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACCTTGAAGGCACTT
TGGAGATTCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGATTCATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GACTCAGAAACATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACCAGCCTTGGCATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTTCGAAATATTTTACTGCGGCTTGGCTTGTCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTCCTGCTGACACCAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGGGTTCTTCCGCTTTTGATTTTTCACTTCCATAGTTTTCAGCCATTATCGATTATCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACG
TTATTTAATCCTTTTTTTATTGTTTTAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAGCCGATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGGAACTGGA
ACTTGAGCATAATAATTGTCCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGGGACAAGGGGACAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGTGTGAGTAAGATAGATATTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTG
AAGGTAAAAGTGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGCGACTTA
GAAGAAGAGGGAGAGGTGCGGTTCCAGGAAGAATATCCTCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCAATCAACCCTAAGATCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACACCCCAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAACTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(23-166)

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Competence protein (Competence)

(203-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

52.797

99.592

0.526
  comEC/celB Streptococcus mitis SK321

45.248

100

0.459
  comEC/celB Streptococcus mitis NCTC 12261

43.817

100

0.443
  comEC/celB Streptococcus pneumoniae Rx1

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae D39

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae R6

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae TIGR4

43.338

100

0.438