Detailed information NTG0918710

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: VNN41_RS07950
• Genome accession: NZ_CP141713
• Coordinates: 1559349..1560053 (+)
• Length: 234 a.a.
• NCBI ID: WP_407350206.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain VC11
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1554349..1565053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN41_RS07930 (VNN41_07920)	-	1554690..1556291 (-)	1602	WP_407350205.1	GH25 family lysozyme	-
VNN41_RS07935 (VNN41_07925)	guaB	1556460..1557941 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
VNN41_RS07940 (VNN41_07930)	rpsU	1558064..1558240 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
VNN41_RS07945 (VNN41_07935)	comGA	1558427..1559371 (+)	945	WP_407350989.1	competence type IV pilus ATPase ComGA	Machinery gene
VNN41_RS07950 (VNN41_07940)	comGB	1559349..1560053 (+)	705	WP_407350206.1	competence type IV pilus assembly protein ComGB	Machinery gene
VNN41_RS07955 (VNN41_07945)	comYC	1560072..1560377 (+)	306	WP_016171026.1	competence type IV pilus major pilin ComGC	Machinery gene
VNN41_RS07960 (VNN41_07950)	comGD	1560352..1560768 (+)	417	WP_003133718.1	competence type IV pilus minor pilin ComGD	Machinery gene
VNN41_RS07965 (VNN41_07955)	comGE	1560734..1561084 (+)	351	WP_270227858.1	competence type IV pilus minor pilin ComGE	-
VNN41_RS07970 (VNN41_07960)	comGF	1561078..1561455 (+)	378	WP_407350990.1	competence type IV pilus minor pilin ComGF	-
VNN41_RS07975 (VNN41_07965)	-	1561688..1562533 (+)	846	WP_003133722.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
VNN41_RS07980 (VNN41_07970)	-	1562619..1563488 (-)	870	WP_407350207.1	RluA family pseudouridine synthase	-

Sequence

Protein

• Length: 234 a.a.
• Molecular weight: 27384.07 Da
• Isoelectric Point: 9.3657

>NTDB_id=918710 VNN41_RS07950 WP_407350206.1 1559349..1560053(+) (comGB) [Lactococcus garvieae strain VC11]
MSLAKQIKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTLFLGCLFSLLVVFYLCRYYWKKKTAL
EAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKEFGFELLQILDRGQKFEDALRLYPIF
TKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLIILIYAAMLLPIYSNMGNLI

Nucleotide

• Length: 705 bp

>NTDB_id=918710 VNN41_RS07950 WP_407350206.1 1559349..1560053(+) (comGB) [Lactococcus garvieae strain VC11]
TTGAGTTTAGCGAAGCAGATTAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCAT
GCTAGGGTTGAAAAATTATTTACTACCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCAA
CCCTCTTTTTAGGGTGCCTCTTCAGTTTACTTGTTGTCTTTTATCTGTGCAGATACTATTGGAAGAAGAAAACTGCTTTA
GAAGCGATGAGGCTAATGGTCAAAATCCCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGA
ATGGGGAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCA
AAGAATTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGACTTTACCCCATATTT
ACCAAGGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGA
AAGCTGGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGT
TGATTATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (104-225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	55.111	96.154	0.53
comGB/cglB	Streptococcus mitis SK321	53.478	98.291	0.526
comGB/cglB	Streptococcus mitis NCTC 12261	53.478	98.291	0.526
comGB/cglB	Streptococcus pneumoniae Rx1	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae TIGR4	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae D39	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae R6	53.043	98.291	0.521
comYB	Streptococcus gordonii str. Challis substr. CH1	52.609	98.291	0.517
comYB	Streptococcus mutans UA140	51.786	95.726	0.496
comYB	Streptococcus mutans UA159	51.339	95.726	0.491