Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   VNN47_RS09325 Genome accession   NZ_CP141709
Coordinates   1839927..1842050 (+) Length   707 a.a.
NCBI ID   WP_407350995.1    Uniprot ID   -
Organism   Lactococcus garvieae strain VC13     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1837852..1839842 1839927..1842050 flank 85


Gene organization within MGE regions


Location: 1837852..1842050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN47_RS09315 (VNN47_09280) istB 1837852..1838610 (-) 759 WP_341485250.1 IS21-like element helper ATPase IstB -
  VNN47_RS09320 (VNN47_09285) istA 1838619..1839842 (-) 1224 WP_341485251.1 IS21 family transposase -
  VNN47_RS09325 (VNN47_09290) comEC 1839927..1842050 (+) 2124 WP_407350995.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene

Sequence

Protein


Download         Length: 707 a.a.        Molecular weight: 81462.31 Da        Isoelectric Point: 9.5946

>NTDB_id=918683 VNN47_RS09325 WP_407350995.1 1839927..1842050(+) (comEC) [Lactococcus garvieae strain VC13]
MLSLFSLFIAFYRQYYSLLALLLCFSLFLLMIKKETEVQERKQPEKISKITPYMDTLQVNGSRLSFRGEVNGNDFQAFYT
LTSEKEKEYFKKLAVKGTFYLEGTLEIPEEQRNFSGFDYRKYLRTQGVYRLLKIEKIHGFEEKQDFDLRLVRRKAILWTQ
EHFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIELLRNILLRLGLSQKLVFFIQIPFSIFYAF
LTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTTGGQLTMLYSFLITFFSGKFKELKGVKKSLLDSTVLA
VGVLPLLIFHFHSFQPLSIFLTLLFSLIFDFLLLPGLLFVFLLSLFGPALTLFNPFFIVLESIVRWVGDLFDQPIIFGKP
NLIFLLLLFMLSGLALDFYKKRNWNLSIIIVLFLLFFFVKNPKHPSITMVDVGQGDSILLQDSWNSQNILIDTGGRIKIR
ADEQWKEGIYKSNAENTLLPYLRARGVSKIDILIVTHPDEDHIGDLESVAQKVPIKNVYVSASALEKKSFAKKLSRLNSQ
IHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDLEEEGEVRLQEEYPHLEVDVLKVGHHGSKT
SSKEAFLEAINPKIALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGAIRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2124 bp        

>NTDB_id=918683 VNN47_RS09325 WP_407350995.1 1839927..1842050(+) (comEC) [Lactococcus garvieae strain VC13]
ATTCTTAGTCTCTTTAGTCTCTTCATCGCTTTTTATCGCCAATATTATAGTTTGTTGGCTCTTTTACTGTGCTTTAGTTT
ATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACCCCTTATA
TGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTTTTACACC
TTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACCTTGAAGGCACTTTGGAGAT
TCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTTCTTAAAA
TAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTGGACTCAG
GAACATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAGAAATGGG
GGAGCTTTACACCAGTCTTGGCATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAACTTCTCC
GAAATATTTTACTGCGGCTTGGCTTATCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTATGCATTT
CTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATAGATTTTC
TGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTTCTGCTGACAACAGGAGGACAACTCACGATGCTTTATTCTT
TCTTAATCACATTTTTTTCAGGGAAGTTCAAAGAACTAAAAGGAGTAAAAAAATCGTTACTTGATTCTACCGTCCTAGCT
GTTGGGGTTCTTCCGCTTTTGATTTTTCACTTTCATAGTTTTCAGCCATTATCGATTTTCTTGACTTTATTATTTAGTCT
GATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTCCTGCTCTCACGTTATTTA
ATCCTTTTTTTATTGTTTTAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAGCCGATTATCTTTGGCAAACCC
AACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGAACTTGAG
CATAATAATTGTTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGATGTGGGAC
AAGGGGATAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGAATAAAAATAAGA
GCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCTGAAAATACACTTCTGCCTTATCTGAGAGCGCGTGGTGT
GAGTAAGATAGATATTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAGAAAGTAC
CAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAACAGTCAG
ATTCACATTGTGCAGGAGGGGGACAAGCTGCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTGAAGGTAA
AAGTGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGCGACTTAGAAGAAG
AGGGAGAGGTGCGGCTCCAGGAAGAATATCCTCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAGTAAAACT
TCTTCAAAGGAAGCTTTTTTAGAAGCAATCAACCCTAAGATCGCTCTTATTTCGGCTGGAAAGAATAATCGATATAAACA
TCCCAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAATTTATCGTACAGACCAGCAAGGGGCAATCCGTC
TCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(7-137)

Metallo-beta-lactamase superfamily (Lactamase_B)

(452-660)

Competence protein (Competence)

(174-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

52.334

100

0.523
  comEC/celB Streptococcus mitis SK321

45.404

100

0.461
  comEC/celB Streptococcus mitis NCTC 12261

44.056

100

0.446
  comEC/celB Streptococcus pneumoniae Rx1

43.454

100

0.441
  comEC/celB Streptococcus pneumoniae D39

43.454

100

0.441
  comEC/celB Streptococcus pneumoniae R6

43.454

100

0.441
  comEC/celB Streptococcus pneumoniae TIGR4

43.557

100

0.44