Detailed information NTG0918666

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: VNN47_RS07955
• Genome accession: NZ_CP141709
• Coordinates: 1559359..1560063 (+)
• Length: 234 a.a.
• NCBI ID: WP_407350206.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain VC13
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1554359..1565063

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN47_RS07935 (VNN47_07900)	-	1554700..1556301 (-)	1602	WP_407350205.1	GH25 family lysozyme	-
VNN47_RS07940 (VNN47_07905)	guaB	1556470..1557951 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
VNN47_RS07945 (VNN47_07910)	rpsU	1558074..1558250 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
VNN47_RS07950 (VNN47_07915)	comGA	1558437..1559381 (+)	945	WP_407350989.1	competence type IV pilus ATPase ComGA	Machinery gene
VNN47_RS07955 (VNN47_07920)	comGB	1559359..1560063 (+)	705	WP_407350206.1	competence type IV pilus assembly protein ComGB	Machinery gene
VNN47_RS07960 (VNN47_07925)	comYC	1560082..1560387 (+)	306	WP_016171026.1	competence type IV pilus major pilin ComGC	Machinery gene
VNN47_RS07965 (VNN47_07930)	comGD	1560362..1560778 (+)	417	WP_003133718.1	competence type IV pilus minor pilin ComGD	Machinery gene
VNN47_RS07970 (VNN47_07935)	comGE	1560744..1561094 (+)	351	WP_270227858.1	competence type IV pilus minor pilin ComGE	-
VNN47_RS07975 (VNN47_07940)	comGF	1561064..1561465 (+)	402	WP_407357374.1	competence type IV pilus minor pilin ComGF	-
VNN47_RS07980 (VNN47_07945)	-	1561698..1562543 (+)	846	WP_003133722.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
VNN47_RS07985 (VNN47_07950)	-	1562629..1563498 (-)	870	WP_407350207.1	RluA family pseudouridine synthase	-

Sequence

Protein

• Length: 234 a.a.
• Molecular weight: 27384.07 Da
• Isoelectric Point: 9.3657

>NTDB_id=918666 VNN47_RS07955 WP_407350206.1 1559359..1560063(+) (comGB) [Lactococcus garvieae strain VC13]
MSLAKQIKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTLFLGCLFSLLVVFYLCRYYWKKKTAL
EAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKEFGFELLQILDRGQKFEDALRLYPIF
TKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLIILIYAAMLLPIYSNMGNLI

Nucleotide

• Length: 705 bp

>NTDB_id=918666 VNN47_RS07955 WP_407350206.1 1559359..1560063(+) (comGB) [Lactococcus garvieae strain VC13]
TTGAGTTTAGCGAAGCAGATTAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCAT
GCTAGGGTTGAAAAATTATTTACTACCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCAA
CCCTCTTTTTAGGGTGCCTCTTCAGTTTACTTGTTGTCTTTTATCTGTGCAGATACTATTGGAAGAAGAAAACTGCTTTA
GAAGCGATGAGGCTAATGGTCAAAATCCCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGA
ATGGGGAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCA
AAGAATTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGACTTTACCCCATATTT
ACCAAGGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGA
AAGCTGGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGT
TGATTATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (104-225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	55.111	96.154	0.53
comGB/cglB	Streptococcus mitis SK321	53.478	98.291	0.526
comGB/cglB	Streptococcus mitis NCTC 12261	53.478	98.291	0.526
comGB/cglB	Streptococcus pneumoniae Rx1	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae TIGR4	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae D39	53.043	98.291	0.521
comGB/cglB	Streptococcus pneumoniae R6	53.043	98.291	0.521
comYB	Streptococcus gordonii str. Challis substr. CH1	52.609	98.291	0.517
comYB	Streptococcus mutans UA140	51.786	95.726	0.496
comYB	Streptococcus mutans UA159	51.339	95.726	0.491