NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	VNN47_RS07950	Genome accession	NZ_CP141709
Coordinates	1558437..1559381 (+)	Length	314 a.a.
NCBI ID	WP_407350989.1	Uniprot ID	-
Organism	Lactococcus garvieae strain VC13
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1553437..1564381

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN47_RS07935 (VNN47_07900)	-	1554700..1556301 (-)	1602	WP_407350205.1	GH25 family lysozyme	-
VNN47_RS07940 (VNN47_07905)	guaB	1556470..1557951 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
VNN47_RS07945 (VNN47_07910)	rpsU	1558074..1558250 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
VNN47_RS07950 (VNN47_07915)	comGA	1558437..1559381 (+)	945	WP_407350989.1	competence type IV pilus ATPase ComGA	Machinery gene
VNN47_RS07955 (VNN47_07920)	comGB	1559359..1560063 (+)	705	WP_407350206.1	competence type IV pilus assembly protein ComGB	Machinery gene
VNN47_RS07960 (VNN47_07925)	comYC	1560082..1560387 (+)	306	WP_016171026.1	competence type IV pilus major pilin ComGC	Machinery gene
VNN47_RS07965 (VNN47_07930)	comGD	1560362..1560778 (+)	417	WP_003133718.1	competence type IV pilus minor pilin ComGD	Machinery gene
VNN47_RS07970 (VNN47_07935)	comGE	1560744..1561094 (+)	351	WP_270227858.1	competence type IV pilus minor pilin ComGE	-
VNN47_RS07975 (VNN47_07940)	comGF	1561064..1561465 (+)	402	WP_407357374.1	competence type IV pilus minor pilin ComGF	-
VNN47_RS07980 (VNN47_07945)	-	1561698..1562543 (+)	846	WP_003133722.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
VNN47_RS07985 (VNN47_07950)	-	1562629..1563498 (-)	870	WP_407350207.1	RluA family pseudouridine synthase	-

Sequence

Protein

Download Length: 314 a.a. Molecular weight: 35823.02 Da Isoelectric Point: 6.5037

                                >NTDB_id=918665 VNN47_RS07950 WP_407350989.1 1558437..1559381(+) (comGA) [Lactococcus garvieae strain VC13]

MIQVLAKTLLQKAVDQMIHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV

LDTGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI

CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR

LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide

Download Length: 945 bp

                                >NTDB_id=918665 VNN47_RS07950 WP_407350989.1 1558437..1559381(+) (comGA) [Lactococcus garvieae strain VC13]

ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGATACATGATCTTTATTTCGTAGCATTAGA

AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC

TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTC

TTAGATACAGGTGAGCAGCGTTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT

GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT

TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAACATTTTAAAAATCAACAAGTTATT

TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA

TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG

CTGTTCTCCGCGCAAGTTTGACGGGCTATACAGTGTTTTCTACTGTTCATGCACGCTCAATATCGGGTGTAATTGCACGG

CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCTAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA

GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG

CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	59.554	100	0.596
comYA	Streptococcus gordonii str. Challis substr. CH1	53.651	100	0.538
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.355	99.682	0.532
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae D39	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae R6	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	53.035	99.682	0.529
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	52.396	99.682	0.522
comYA	Streptococcus mutans UA159	51.603	99.363	0.513
comYA	Streptococcus mutans UA140	51.603	99.363	0.513

Detailed information NTG0918665