Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VNN44_RS03170 Genome accession   NZ_CP141689
Coordinates   641232..641498 (-) Length   88 a.a.
NCBI ID   WP_255566449.1    Uniprot ID   -
Organism   Lactococcus petauri strain R22-16 FS-A5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636232..646498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN44_RS03155 (VNN44_03155) - 636836..639124 (-) 2289 WP_019335999.1 PBP1A family penicillin-binding protein -
  VNN44_RS03160 (VNN44_03160) - 639200..640084 (+) 885 WP_019336000.1 RluA family pseudouridine synthase -
  VNN44_RS03165 (VNN44_03165) - 640155..641000 (-) 846 WP_019336001.1 zinc ABC transporter substrate-binding protein -
  VNN44_RS03170 (VNN44_03170) comYF 641232..641498 (-) 267 WP_255566449.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN44_RS03175 (VNN44_03175) comGE 641653..641817 (-) 165 WP_019336003.1 competence system putative prepilin ComGE -
  VNN44_RS03180 (VNN44_03180) - 641790..641888 (-) 99 WP_255566393.1 hypothetical protein -
  VNN44_RS03185 (VNN44_03185) comGD 641920..642336 (-) 417 WP_019336004.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN44_RS03190 (VNN44_03190) comYC 642311..642613 (-) 303 WP_346349212.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN44_RS03195 (VNN44_03195) comGB 642632..643651 (-) 1020 WP_255566448.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN44_RS03200 (VNN44_03200) comGA 643590..644534 (-) 945 WP_195916935.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN44_RS03205 (VNN44_03205) rpsU 644720..644896 (-) 177 WP_003134539.1 30S ribosomal protein S21 -

Sequence

Protein


Download         Length: 88 a.a.        Molecular weight: 10169.57 Da        Isoelectric Point: 10.1554

>NTDB_id=918176 VNN44_RS03170 WP_255566449.1 641232..641498(-) (comYF) [Lactococcus petauri strain R22-16 FS-A5]
MRSELEGASLDKVENNYLYVRKNVNLRFGLSSKGDFRKTNANGRGYQPMIHHVKNVKISQEGEQVKIILSFEKGGDRIFL
YKFSEKES

Nucleotide


Download         Length: 267 bp        

>NTDB_id=918176 VNN44_RS03170 WP_255566449.1 641232..641498(-) (comYF) [Lactococcus petauri strain R22-16 FS-A5]
CTGAGAAGCGAACTTGAGGGAGCAAGCTTAGATAAGGTGGAAAATAACTATCTTTATGTCCGCAAAAATGTCAATCTAAG
ATTTGGACTTTCCTCCAAAGGAGATTTTCGGAAAACTAATGCTAATGGGCGAGGTTATCAACCCATGATCCATCATGTAA
AAAATGTTAAAATCAGCCAGGAGGGAGAGCAGGTAAAAATTATCTTGTCTTTTGAAAAAGGAGGAGACCGCATATTTCTT
TACAAGTTCTCAGAAAAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.724

98.864

0.511
  comYF Streptococcus mutans UA159

50.575

98.864

0.5
  comGF Lactococcus lactis subsp. cremoris KW2

50.588

96.591

0.489
  comGF/cglF Streptococcus pneumoniae TIGR4

39.326

100

0.398
  comGF/cglF Streptococcus pneumoniae D39

39.326

100

0.398
  comGF/cglF Streptococcus pneumoniae Rx1

39.326

100

0.398
  comGF/cglF Streptococcus pneumoniae R6

39.326

100

0.398
  comGF/cglF Streptococcus mitis NCTC 12261

35.955

100

0.364