Detailed information NTG0917971

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U9S62_RS28705
• Genome accession: NZ_CP141683
• Coordinates: 6149471..6150619 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZM21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6144471..6155619

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U9S62_RS28685 (U9S62_28685)	-	6145209..6146393 (-)	1185	WP_014602386.1	cystathionine gamma-synthase	-
U9S62_RS28690 (U9S62_28690)	-	6146390..6147763 (-)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
U9S62_RS28695 (U9S62_28695)	-	6148006..6148410 (-)	405	WP_003084560.1	NINE protein	-
U9S62_RS28700 (U9S62_28700)	czcD	6148564..6149463 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
U9S62_RS28705 (U9S62_28705)	pilU	6149471..6150619 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
U9S62_RS28710 (U9S62_28710)	pilT	6150797..6151831 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
U9S62_RS28715 (U9S62_28715)	-	6152046..6152738 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
U9S62_RS28720 (U9S62_28720)	proC	6152750..6153571 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
U9S62_RS28725 (U9S62_28725)	-	6153582..6154175 (+)	594	WP_003084544.1	YggT family protein	-
U9S62_RS28730 (U9S62_28730)	-	6154172..6154468 (+)	297	WP_031628073.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=917971 U9S62_RS28705 WP_003100959.1 6149471..6150619(-) (pilU) [Pseudomonas aeruginosa strain ZM21]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=917971 U9S62_RS28705 WP_003100959.1 6149471..6150619(-) (pilU) [Pseudomonas aeruginosa strain ZM21]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382