Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV19_RS01255 Genome accession   NZ_AP025463
Coordinates   279818..280843 (+) Length   341 a.a.
NCBI ID   WP_065676550.1    Uniprot ID   A0A1C3JEM3
Organism   Vibrio celticus strain CECT 7224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274818..285843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV19_RS01235 - 275150..275878 (-) 729 WP_017061133.1 glutathione peroxidase -
  OCV19_RS01240 - 275923..276102 (-) 180 WP_048659170.1 hypothetical protein -
  OCV19_RS01245 oxyR 276101..276994 (+) 894 WP_017061132.1 DNA-binding transcriptional regulator OxyR -
  OCV19_RS01250 - 277105..279666 (-) 2562 WP_065676551.1 penicillin-binding protein 1A -
  OCV19_RS01255 pilM 279818..280843 (+) 1026 WP_065676550.1 type IV pilus assembly protein PilM Machinery gene
  OCV19_RS01260 pilN 280827..281426 (+) 600 WP_065676549.1 PilN domain-containing protein Machinery gene
  OCV19_RS01265 pilO 281419..282048 (+) 630 WP_065676548.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV19_RS01270 pilP 282035..282553 (+) 519 WP_065676547.1 pilus assembly protein PilP Machinery gene
  OCV19_RS01275 pilQ 282589..284307 (+) 1719 WP_065676546.1 type IV pilus secretin PilQ Machinery gene
  OCV19_RS01280 aroK 284484..285002 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38287.71 Da        Isoelectric Point: 6.4182

>NTDB_id=91680 OCV19_RS01255 WP_065676550.1 279818..280843(+) (pilM) [Vibrio celticus strain CECT 7224]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKKNWLLVDVGVDQTSLGIIPQNSAPFYKDIAYGTQDLRSSDDPSDIESVFGTMEETH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91680 OCV19_RS01255 WP_065676550.1 279818..280843(+) (pilM) [Vibrio celticus strain CECT 7224]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACA
GTGATAAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAAGAAGTGGTGGATAGCCGAGCGGATGCATTAACCAAGGCTGGCTTCAAACCT
GTGGTGGTGGATACACAGGCGCATGGGCTGCTGAATATCTGGCAATTGGCCTCGCGCCTGTATCCCGATAAGAAAAACTG
GTTGCTGGTGGATGTTGGAGTCGACCAAACTTCGCTAGGGATTATCCCGCAGAATTCGGCGCCTTTCTATAAAGACATCG
CCTATGGCACTCAAGATCTTCGCAGCTCAGATGACCCGAGTGATATCGAGAGCGTGTTCGGTACTATGGAAGAGACACAT
CAATTTATCGTTAACCTGATTGAGAAGCTCAAGCGTCAATTACAGCTCTATTCATCGGGAAATGCGCAGCAACCTATTTC
TGGGATTTGGTTGATGGGCGAGGGCGCCAGTATTCCTATGGTTACCGAAGAGCTAGAACGTCATTTTCAGCTGAGCTGTG
AGTCATTGAATCCTTTGTCTCTGTTTGAGAATAAGGTCGCCAAGAGACGCCGACTGCCGATGGACTGGCAACACTTTGGT
ATCGCGGCGGGTATGGCGATGAGTGGGCTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3JEM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.839

100

0.548
  pilM Vibrio campbellii strain DS40M4

48.688

100

0.49

Multiple sequence alignment