Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCV30_RS14205 Genome accession   NZ_AP025460
Coordinates   3139834..3140448 (-) Length   204 a.a.
NCBI ID   WP_065679726.1    Uniprot ID   A0A1C3IY48
Organism   Vibrio atlanticus strain CECT 7223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3134834..3145448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV30_RS14180 aroB 3135145..3136233 (-) 1089 WP_009848908.1 3-dehydroquinate synthase -
  OCV30_RS14185 aroK 3136258..3136776 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OCV30_RS14190 pilQ 3136953..3138671 (-) 1719 WP_017103612.1 type IV pilus secretin PilQ Machinery gene
  OCV30_RS14195 pilP 3138707..3139225 (-) 519 WP_065679725.1 pilus assembly protein PilP Machinery gene
  OCV30_RS14200 pilO 3139212..3139841 (-) 630 WP_012604947.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV30_RS14205 pilN 3139834..3140448 (-) 615 WP_065679726.1 PilN domain-containing protein Machinery gene
  OCV30_RS14210 pilM 3140432..3141457 (-) 1026 WP_065679727.1 type IV pilus assembly protein PilM Machinery gene
  OCV30_RS14215 - 3141609..3144167 (+) 2559 WP_065679728.1 penicillin-binding protein 1A -
  OCV30_RS14220 oxyR 3144276..3145169 (-) 894 WP_065679729.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23398.84 Da        Isoelectric Point: 9.3205

>NTDB_id=91664 OCV30_RS14205 WP_065679726.1 3139834..3140448(-) (pilN) [Vibrio atlanticus strain CECT 7223]
MLHQINLLPWRDEIRAQHKKRFIHLVILGVIIALTGQWAVGHYFQDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMAGISDSTARLATMLDNLERSDSLKGVEMHSII
HNRKRFNKEFQTFKVSFVFSPVSLENTTAESATASRKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=91664 OCV30_RS14205 WP_065679726.1 3139834..3140448(-) (pilN) [Vibrio atlanticus strain CECT 7223]
ATGTTGCATCAAATTAACCTGCTGCCGTGGCGTGATGAGATTCGCGCTCAGCACAAGAAGCGTTTTATTCACCTAGTCAT
TCTTGGCGTCATCATTGCACTTACAGGGCAATGGGCGGTTGGTCACTATTTTCAAGATCAACAAGCCAAACAGCAAGCTC
GCCTCAATTATCTTAATCAATACATTGCCGAGCTCGACCGACAAATTCAATCGTTAAAAGTTGCAGAACAAGAACACAAA
GCCATTCTGACTCGACTTGATGTCGTGGAGTCGTTACAGCTTGGCCGTAATAAGACCACCGACTTTATGAATCTAATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAGATGGCAGGTATCAGCGACA
GTACGGCTCGCCTCGCCACAATGTTGGACAATCTAGAGCGTTCTGATTCTCTTAAAGGGGTCGAGATGCACTCTATTATC
CATAACCGAAAGCGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACCGCAGAGAGCGCAACCGCGAGCAGAAAAGAGAAGGGGGCAAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3IY48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

65.217

90.196

0.588
  pilN Vibrio campbellii strain DS40M4

62.903

91.176

0.574

Multiple sequence alignment