Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV30_RS14210 Genome accession   NZ_AP025460
Coordinates   3140432..3141457 (-) Length   341 a.a.
NCBI ID   WP_065679727.1    Uniprot ID   A0A1C3IYI4
Organism   Vibrio atlanticus strain CECT 7223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3135432..3146457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV30_RS14185 aroK 3136258..3136776 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OCV30_RS14190 pilQ 3136953..3138671 (-) 1719 WP_017103612.1 type IV pilus secretin PilQ Machinery gene
  OCV30_RS14195 pilP 3138707..3139225 (-) 519 WP_065679725.1 pilus assembly protein PilP Machinery gene
  OCV30_RS14200 pilO 3139212..3139841 (-) 630 WP_012604947.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV30_RS14205 pilN 3139834..3140448 (-) 615 WP_065679726.1 PilN domain-containing protein Machinery gene
  OCV30_RS14210 pilM 3140432..3141457 (-) 1026 WP_065679727.1 type IV pilus assembly protein PilM Machinery gene
  OCV30_RS14215 - 3141609..3144167 (+) 2559 WP_065679728.1 penicillin-binding protein 1A -
  OCV30_RS14220 oxyR 3144276..3145169 (-) 894 WP_065679729.1 DNA-binding transcriptional regulator OxyR -
  OCV30_RS14225 - 3145391..3146119 (+) 729 WP_065679730.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38187.67 Da        Isoelectric Point: 6.3428

>NTDB_id=91665 OCV30_RS14210 WP_065679727.1 3140432..3141457(-) (pilM) [Vibrio atlanticus strain CECT 7223]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESDLEDREKEFSIYQTFAHQSPFPIEDLSLDFVKLEDKRFGKGSTSSYQIYATRKEVVDSRADALNKAGFKP
VVIDTQAHGLLNIWQLASRLYPEKSNWLLVDVGLDQTSLGIIPQDSAPFYKDIAFGTQDLRGTDCPSYTEGVFGNAEETH
RFIVNLVEKLKRQLQLYSSVNALEPISGIWLMGEGACIPMVAEDLERHFQMSCESLNPLSLFENKVAKRHQLPMNWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91665 OCV30_RS14210 WP_065679727.1 3140432..3141457(-) (pilM) [Vibrio atlanticus strain CECT 7223]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCCGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGACTTAGAAGACAGAGAAAAGGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGATCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGATCGACAA
GTAGCTATCAGATATATGCGACTCGTAAGGAAGTTGTAGACAGTCGAGCAGATGCTTTAAATAAAGCTGGGTTTAAACCT
GTTGTCATCGACACCCAAGCACATGGTCTGCTCAATATTTGGCAGTTGGCTTCACGCCTTTATCCTGAAAAAAGTAACTG
GTTGCTGGTGGACGTGGGACTTGACCAAACGTCGTTAGGCATTATCCCGCAGGATTCAGCACCTTTCTATAAAGACATCG
CCTTTGGTACTCAAGATCTTCGTGGCACTGACTGTCCGAGTTATACAGAAGGTGTGTTCGGCAACGCAGAGGAGACACAT
CGGTTTATTGTCAATTTGGTTGAAAAGCTTAAGCGACAGTTGCAACTCTATTCCTCAGTGAATGCACTTGAGCCGATATC
CGGGATCTGGCTGATGGGCGAAGGAGCCTGTATTCCGATGGTGGCTGAAGATCTAGAGCGCCATTTTCAGATGAGTTGTG
AATCGCTCAATCCTTTATCGTTATTTGAGAACAAGGTGGCGAAGCGGCACCAACTGCCGATGAACTGGCAACATTTCGGC
ATTGCCGCGGGTATGGCGATGAGTGGACTCAAGTGGCAAGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3IYI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.666

100

0.537
  pilM Vibrio campbellii strain DS40M4

46.784

100

0.469

Multiple sequence alignment