Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   SPJ73_RS09925 Genome accession   NZ_CP140795
Coordinates   2108352..2109686 (+) Length   444 a.a.
NCBI ID   WP_250023519.1    Uniprot ID   -
Organism   Pasteurella multocida strain PMLO     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2103352..2114686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPJ73_RS09900 (SPJ73_09885) - 2103428..2105989 (-) 2562 WP_005754757.1 penicillin-binding protein 1A -
  SPJ73_RS09905 (SPJ73_09890) - 2106125..2106898 (+) 774 WP_104884244.1 pilus assembly protein PilM -
  SPJ73_RS09910 (SPJ73_09895) - 2106938..2107453 (+) 516 WP_083003385.1 competence protein ComB -
  SPJ73_RS09915 (SPJ73_09900) - 2107453..2107977 (+) 525 WP_096743656.1 ATPase -
  SPJ73_RS09920 (SPJ73_09905) - 2107980..2108342 (+) 363 WP_250023517.1 pilus assembly protein PilP -
  SPJ73_RS09925 (SPJ73_09910) comE 2108352..2109686 (+) 1335 WP_250023519.1 type IV pilus secretin PilQ Machinery gene
  SPJ73_RS09930 (SPJ73_09915) aroK 2109869..2110396 (+) 528 WP_005717623.1 shikimate kinase AroK -
  SPJ73_RS09935 (SPJ73_09920) aroB 2110413..2111501 (+) 1089 WP_250023521.1 3-dehydroquinate synthase -
  SPJ73_RS09940 (SPJ73_09925) - 2111505..2112410 (+) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  SPJ73_RS09945 (SPJ73_09930) - 2112458..2112763 (-) 306 WP_115300063.1 DciA family protein -
  SPJ73_RS09950 (SPJ73_09935) secM 2112856..2113167 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49236.60 Da        Isoelectric Point: 7.3923

>NTDB_id=915639 SPJ73_RS09925 WP_250023519.1 2108352..2109686(+) (comE) [Pasteurella multocida strain PMLO]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALRQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSSSGVAANDTHHPTSLMTSSIKLHFAKAADVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSLPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=915639 SPJ73_RS09925 WP_250023519.1 2108352..2109686(+) (comE) [Pasteurella multocida strain PMLO]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCGACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTGGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCTCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGATGTAGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTAGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTAGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTTGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.124

100

0.613
  comE Haemophilus influenzae 86-028NP

60.449

100

0.606
  comE Glaesserella parasuis strain SC1401

49.173

95.27

0.468
  pilQ Vibrio campbellii strain DS40M4

39.762

94.595

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.049

92.793

0.372
  pilQ Vibrio cholerae strain A1552

40.049

92.793

0.372