Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U6037_RS12815 Genome accession   NZ_CP140706
Coordinates   2911587..2912696 (+) Length   369 a.a.
NCBI ID   WP_322847012.1    Uniprot ID   -
Organism   Pseudomonas sp. B33.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2906587..2917696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6037_RS12800 - 2907564..2908502 (+) 939 WP_322847010.1 sugar kinase -
  U6037_RS12805 - 2908609..2908884 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  U6037_RS12810 - 2908936..2911473 (-) 2538 WP_322847011.1 PAS domain S-box protein -
  U6037_RS12815 pilU 2911587..2912696 (+) 1110 WP_322847012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U6037_RS12820 - 2912840..2916532 (+) 3693 WP_322847013.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40723.55 Da        Isoelectric Point: 6.7833

>NTDB_id=915034 U6037_RS12815 WP_322847012.1 2911587..2912696(+) (pilU) [Pseudomonas sp. B33.4]
MEIDALLSVLSNKNGSDLFLSTGASPSARIDGVLTALSDQTFKNGETAAIAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPAVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAINANQALDRI
INMFPQERRPQLLQALGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTIADSIRRNELGELKSIMEKSAEAGMQTFDTAL
LRLVEERAITVDEALKNADSVNNLRLGIKLKVESAGNADMSTGDWGLVE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=915034 U6037_RS12815 WP_322847012.1 2911587..2912696(+) (pilU) [Pseudomonas sp. B33.4]
ATGGAAATCGATGCACTGTTGTCAGTTTTGTCCAATAAAAACGGTTCCGACCTGTTCCTCTCCACGGGTGCGTCGCCGAG
CGCACGCATCGACGGCGTGCTGACAGCGCTGAGCGATCAAACCTTCAAGAACGGTGAAACCGCCGCGATAGCAGCTTCCC
TGATGGACGCCGAGCAACGACGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAACGGGCATCGGCCGC
TTTCGCGTGAACATCTTCAAACAGCGCAACGATGTATCGATCGTCATACGCAACGTCAAACTCGACATTCCGCGTTTTGC
CGATTTGAAACTGCCGGCGGTACTGCTCGAAACCGTCATGCTCAAGCAAGGGTTGATCCTGTTCGTCGGCGCCACCGATT
CCGGTAAATCAACCTCGCTGGCCGCGCTGATCGACCACCGCAACCGCCACAGCAGCGGCCACATCATCACCATCGAGGAT
CCGATCGAGTACATCCATCGGCATCAGCGTTCGATCATCAATCAACGGGAAGTCGGGGTCGATACGCGCAGCTTCCACGC
AGCCTTGAAAAACACCCTGCGCCAGGCACCGGATGTAGTGCTGATTGGTGAAATCCGCGACCGCGAAACCATGGAACATG
CTTTAGCGTTTGCCGATACCGGGCATCTGGTGTTGTCGACGTTGCATGCCATCAATGCCAATCAAGCGTTGGACCGGATT
ATCAATATGTTCCCGCAAGAGCGCCGGCCGCAGTTGTTGCAGGCGCTGGGCAATAATTTGAAAGCATTTATCTCGCAACG
CTTGGTGCGCACTGTTGATGGCCAACGAAGGGCGGCTGTAGAGGTGTTATTGGGCACCCCGACAATTGCTGATTCGATCC
GACGCAATGAGTTAGGCGAATTGAAAAGTATCATGGAAAAATCCGCAGAGGCGGGCATGCAGACTTTCGATACAGCACTG
CTACGGTTGGTCGAAGAGCGTGCGATTACAGTGGATGAGGCGTTAAAAAATGCAGACTCGGTAAATAATTTAAGGCTTGG
TATAAAGCTCAAAGTCGAGTCCGCAGGGAATGCTGATATGTCTACAGGTGATTGGGGTTTGGTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.462

96.748

0.537
  pilU Acinetobacter baylyi ADP1

53.652

96.477

0.518
  pilU Vibrio cholerae strain A1552

50.568

95.393

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369