Detailed information NTG0914637

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U4X11_RS02610
• Genome accession: NZ_CP140515
• Coordinates: 571533..572681 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2023CK-01480
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 566533..577681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U4X11_RS02585 (U4X11_02590)	-	567684..567980 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
U4X11_RS02590 (U4X11_02595)	-	567977..568570 (-)	594	WP_003084544.1	YggT family protein	-
U4X11_RS02595 (U4X11_02600)	proC	568581..569402 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
U4X11_RS02600 (U4X11_02605)	-	569414..570106 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
U4X11_RS02605 (U4X11_02610)	pilT	570321..571355 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
U4X11_RS02610 (U4X11_02615)	pilU	571533..572681 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
U4X11_RS02615 (U4X11_02620)	czcD	572689..573588 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
U4X11_RS02620 (U4X11_02625)	-	573743..574147 (+)	405	WP_003084560.1	NINE protein	-
U4X11_RS02625 (U4X11_02630)	-	574390..575763 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
U4X11_RS02630 (U4X11_02635)	-	575760..576944 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=914637 U4X11_RS02610 WP_003110016.1 571533..572681(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01480]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=914637 U4X11_RS02610 WP_003110016.1 571533..572681(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01480]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382