Detailed information NTG0914287

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U2S94_RS15445
• Genome accession: NZ_CP140434
• Coordinates: 3045383..3046501 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 2023CK-01274
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3040383..3051501

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2S94_RS15435 (U2S94_15455)	-	3043499..3044191 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
U2S94_RS15440 (U2S94_15460)	pilT	3044318..3045355 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
U2S94_RS15445 (U2S94_15465)	pilU	3045383..3046501 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U2S94_RS15450 (U2S94_15470)	fur	3046571..3047008 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
U2S94_RS15455 (U2S94_15475)	-	3047120..3047518 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
U2S94_RS15460 (U2S94_15480)	-	3047573..3047896 (-)	324	WP_000442587.1	RnfH family protein	-
U2S94_RS15465 (U2S94_15485)	-	3047896..3048963 (-)	1068	WP_002019572.1	hypothetical protein	-
U2S94_RS15470 (U2S94_15490)	-	3049116..3049571 (-)	456	WP_000782976.1	bacteriohemerythrin	-
U2S94_RS15475 (U2S94_15495)	-	3049824..3050672 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=914287 U2S94_RS15445 WP_000347039.1 3045383..3046501(+) (pilU) [Acinetobacter baumannii strain 2023CK-01274]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=914287 U2S94_RS15445 WP_000347039.1 3045383..3046501(+) (pilU) [Acinetobacter baumannii strain 2023CK-01274]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395