Detailed information NTG0914226

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U2S93_RS02025
• Genome accession: NZ_CP140431
• Coordinates: 429409..430557 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2023CK-01249
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 424409..435557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2S93_RS02000 (U2S93_02000)	-	425560..425856 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
U2S93_RS02005 (U2S93_02005)	-	425853..426446 (-)	594	WP_003084544.1	YggT family protein	-
U2S93_RS02010 (U2S93_02010)	proC	426457..427278 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
U2S93_RS02015 (U2S93_02015)	-	427290..427982 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
U2S93_RS02020 (U2S93_02020)	pilT	428197..429231 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
U2S93_RS02025 (U2S93_02025)	pilU	429409..430557 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
U2S93_RS02030 (U2S93_02030)	czcD	430565..431464 (-)	900	WP_022580619.1	CDF family zinc transporter CzcD	-
U2S93_RS02035 (U2S93_02035)	-	431618..432022 (+)	405	WP_003084560.1	NINE protein	-
U2S93_RS02040 (U2S93_02040)	-	432265..433638 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
U2S93_RS02045 (U2S93_02045)	-	433635..434819 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=914226 U2S93_RS02025 WP_003110016.1 429409..430557(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01249]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=914226 U2S93_RS02025 WP_003110016.1 429409..430557(+) (pilU) [Pseudomonas aeruginosa strain 2023CK-01249]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTACTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382