Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SR900_RS03725 Genome accession   NZ_CP140158
Coordinates   770872..771939 (+) Length   355 a.a.
NCBI ID   WP_018624023.1    Uniprot ID   -
Organism   Kangiella aquimarina strain DSMZ 16071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765872..776939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR900_RS03715 (SR900_03715) rpmE 767685..767894 (+) 210 WP_018624021.1 50S ribosomal protein L31 -
  SR900_RS03720 (SR900_03720) - 767987..770482 (-) 2496 WP_228127059.1 penicillin-binding protein 1A -
  SR900_RS03725 (SR900_03725) pilM 770872..771939 (+) 1068 WP_018624023.1 pilus assembly protein PilM Machinery gene
  SR900_RS03730 (SR900_03730) - 771939..772514 (+) 576 WP_018624024.1 PilN domain-containing protein -
  SR900_RS03735 (SR900_03735) - 772514..773143 (+) 630 WP_018624025.1 type 4a pilus biogenesis protein PilO -
  SR900_RS03740 (SR900_03740) - 773140..773700 (+) 561 WP_033413822.1 pilus assembly protein PilP -
  SR900_RS03745 (SR900_03745) pilQ 773712..775802 (+) 2091 WP_018624027.1 type IV pilus secretin PilQ -
  SR900_RS03750 (SR900_03750) aroK 775899..776423 (+) 525 WP_018624028.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38661.97 Da        Isoelectric Point: 4.4582

>NTDB_id=913159 SR900_RS03725 WP_018624023.1 770872..771939(+) (pilM) [Kangiella aquimarina strain DSMZ 16071]
MVLGLFKKKQVPVIGIDISSTAVKVLQLGKVGNRYRVDRYAVEPLPQGVVVERDIRDTEAVGNAIKKAVDKARTTTKYAA
VAVSGSAVITRSIQMEKGLTDQEMEDVIKVEADQYIPYPLEEVNLDFEVLGDSDVNDNQVDVLLAASRSVNVDSRVDALE
IAGLIPKVVDIEAYAIERAYQLTKKKTVEHIENSVVAIVDIGATMTSLNVLRNGQVIYNREQLFGGNQLTEEIQSRYGLS
YQEAGLAKKQGSLPDDYETEVLIPFAEIITQQVGRALQFFFSASEFNSVNEIILAGGCSSIDGLDGMIQEHLGTPTRVAN
PFAEMSISSHVNTQALSADAPAMMISCGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=913159 SR900_RS03725 WP_018624023.1 770872..771939(+) (pilM) [Kangiella aquimarina strain DSMZ 16071]
ATGGTTCTGGGGTTATTCAAAAAGAAACAAGTACCAGTCATCGGTATCGATATCAGCTCGACAGCTGTTAAGGTACTCCA
GCTTGGCAAAGTGGGGAATCGTTACAGAGTTGATCGTTATGCGGTAGAACCACTACCACAAGGTGTGGTGGTTGAGCGCG
ATATTCGTGATACTGAAGCCGTCGGCAATGCAATCAAGAAAGCTGTCGATAAGGCTCGTACAACAACAAAATATGCAGCC
GTGGCCGTTTCTGGTTCCGCGGTTATCACTCGTTCTATTCAGATGGAAAAGGGACTGACCGACCAGGAGATGGAAGACGT
CATCAAGGTCGAAGCAGATCAATATATACCTTATCCATTGGAAGAGGTTAATCTTGACTTTGAGGTCTTGGGTGATTCAG
ATGTGAATGATAACCAGGTTGACGTACTATTAGCAGCGTCTCGAAGCGTGAACGTAGATTCTCGTGTTGATGCACTGGAA
ATTGCGGGCCTGATTCCAAAAGTGGTGGATATTGAGGCTTACGCCATTGAGCGTGCTTATCAGTTAACCAAAAAGAAGAC
CGTTGAGCATATCGAAAACTCGGTAGTGGCCATTGTGGATATCGGTGCCACCATGACCAGCTTAAATGTTTTGCGTAATG
GACAGGTTATTTATAACCGCGAGCAGTTATTTGGTGGTAACCAACTAACTGAGGAAATTCAAAGCCGTTACGGTTTGTCA
TATCAAGAAGCTGGCTTGGCGAAAAAGCAGGGTAGCTTGCCTGATGATTACGAAACAGAAGTTCTGATTCCATTCGCGGA
AATTATAACCCAGCAAGTGGGGCGCGCCTTACAGTTCTTCTTCTCTGCGAGCGAATTTAATTCTGTTAATGAAATCATTT
TGGCGGGCGGTTGTTCCTCAATTGATGGCCTTGATGGCATGATTCAGGAGCACCTGGGAACCCCAACACGGGTTGCTAAC
CCATTTGCTGAAATGTCGATTTCTTCCCATGTGAATACTCAGGCTTTAAGTGCGGATGCTCCAGCGATGATGATTAGCTG
TGGACTAGCACTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

99.718

0.499
  comM Acinetobacter nosocomialis M2

49.718

99.718

0.496
  comM Acinetobacter baylyi ADP1

47.175

99.718

0.47
  pilM Legionella pneumophila strain ERS1305867

46.328

99.718

0.462