Detailed information NTG0912604

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: URF95_RS02295
• Genome accession: NZ_CP140111
• Coordinates: 474614..475762 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 19-3158
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 469614..480762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
URF95_RS02270 (URF95_02270)	-	470765..471061 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
URF95_RS02275 (URF95_02275)	-	471058..471651 (-)	594	WP_003084544.1	YggT family protein	-
URF95_RS02280 (URF95_02280)	proC	471662..472483 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
URF95_RS02285 (URF95_02285)	-	472495..473187 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
URF95_RS02290 (URF95_02290)	pilT	473402..474436 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
URF95_RS02295 (URF95_02295)	pilU	474614..475762 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
URF95_RS02300 (URF95_02300)	czcD	475770..476669 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
URF95_RS02305 (URF95_02305)	-	476824..477228 (+)	405	WP_003084560.1	NINE protein	-
URF95_RS02310 (URF95_02310)	-	477471..478844 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
URF95_RS02315 (URF95_02315)	-	478841..480025 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=912604 URF95_RS02295 WP_003100959.1 474614..475762(+) (pilU) [Pseudomonas aeruginosa strain 19-3158]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=912604 URF95_RS02295 WP_003100959.1 474614..475762(+) (pilU) [Pseudomonas aeruginosa strain 19-3158]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382