Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ABQ274_RS10555 Genome accession   NZ_CP159281
Coordinates   2106484..2106930 (+) Length   148 a.a.
NCBI ID   WP_031558968.1    Uniprot ID   -
Organism   Lactococcus lactis strain FNZ339     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2101484..2111930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ274_RS10515 (ABQ274_10515) - 2103013..2103243 (+) 231 WP_129300312.1 hypothetical protein -
  ABQ274_RS10520 (ABQ274_10520) - 2103256..2103606 (+) 351 WP_023164508.1 hypothetical protein -
  ABQ274_RS10525 (ABQ274_10525) - 2103619..2103906 (+) 288 WP_023164507.1 phage holin -
  ABQ274_RS10530 (ABQ274_10530) - 2103906..2104685 (+) 780 WP_353726892.1 peptidoglycan amidohydrolase family protein -
  ABQ274_RS10535 (ABQ274_10535) - 2104765..2105487 (+) 723 WP_353726893.1 hypothetical protein -
  ABQ274_RS10540 (ABQ274_10540) comGC 2105593..2105862 (+) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  ABQ274_RS10545 (ABQ274_10545) comGD 2105855..2106253 (+) 399 WP_023164614.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABQ274_RS10550 (ABQ274_10550) comGE 2106225..2106521 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABQ274_RS10555 (ABQ274_10555) comGF 2106484..2106930 (+) 447 WP_031558968.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABQ274_RS10560 (ABQ274_10560) comGG 2106969..2107253 (+) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABQ274_RS10565 (ABQ274_10565) - 2107334..2107771 (+) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  ABQ274_RS10570 (ABQ274_10570) - 2107768..2108610 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ABQ274_RS10575 (ABQ274_10575) - 2108787..2109524 (+) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  ABQ274_RS10580 (ABQ274_10580) - 2109517..2110326 (+) 810 WP_010906310.1 metal ABC transporter permease -
  ABQ274_RS10585 (ABQ274_10585) - 2110365..2111231 (-) 867 WP_353726894.1 RluA family pseudouridine synthase -
  ABQ274_RS10590 (ABQ274_10590) - 2111436..2111813 (+) 378 WP_003129983.1 pyridoxamine 5'-phosphate oxidase family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17069.78 Da        Isoelectric Point: 8.2389

>NTDB_id=911198 ABQ274_RS10555 WP_031558968.1 2106484..2106930(+) (comGF) [Lactococcus lactis strain FNZ339]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSINDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=911198 ABQ274_RS10555 WP_031558968.1 2106484..2106930(+) (comGF) [Lactococcus lactis strain FNZ339]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
ATGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

74.82

93.919

0.703
  comGF Streptococcus mutans UA140

47.518

95.27

0.453
  comGF Streptococcus mutans UA159

46.809

95.27

0.446
  comGF/cglF Streptococcus mitis SK321

42.336

92.568

0.392
  comGF/cglF Streptococcus mitis NCTC 12261

40.876

92.568

0.378
  comGF/cglF Streptococcus pneumoniae Rx1

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae D39

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae R6

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae TIGR4

39.716

95.27

0.378