Detailed information NTG0911187

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U0543_RS06525
• Genome accession: NZ_CP139836
• Coordinates: 1343249..1344367 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain B84
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1338249..1349367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0543_RS06515 (U0543_06515)	-	1341365..1342057 (-)	693	WP_032050220.1	YggS family pyridoxal phosphate-dependent enzyme	-
U0543_RS06520 (U0543_06520)	pilT	1342184..1343221 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
U0543_RS06525 (U0543_06525)	pilU	1343249..1344367 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U0543_RS06530 (U0543_06530)	fur	1344437..1344874 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
U0543_RS06535 (U0543_06535)	-	1344986..1345384 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
U0543_RS06540 (U0543_06540)	-	1345439..1345762 (-)	324	WP_000442589.1	RnfH family protein	-
U0543_RS06545 (U0543_06545)	-	1345762..1346829 (-)	1068	WP_031962994.1	membrane protein	-
U0543_RS06550 (U0543_06550)	-	1346982..1347437 (-)	456	WP_000782976.1	bacteriohemerythrin	-
U0543_RS06555 (U0543_06555)	-	1347690..1348538 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=911187 U0543_RS06525 WP_000347036.1 1343249..1344367(+) (pilU) [Acinetobacter baumannii strain B84]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=911187 U0543_RS06525 WP_000347036.1 1343249..1344367(+) (pilU) [Acinetobacter baumannii strain B84]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392