Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ABU997_RS07160 Genome accession   NZ_CP159152
Coordinates   1169281..1171479 (+) Length   732 a.a.
NCBI ID   WP_001324365.1    Uniprot ID   -
Organism   Escherichia coli strain 10035210-2     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1164281..1176479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU997_RS07145 (ABU997_07150) - 1164288..1164611 (-) 324 WP_042032467.1 RHS element protein dsORF-f3 -
  ABU997_RS07150 (ABU997_07155) - 1164613..1168706 (-) 4094 Protein_1078 RHS repeat-associated core domain-containing protein -
  ABU997_RS07155 (ABU997_07160) rpmE 1168866..1169078 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  ABU997_RS07160 (ABU997_07165) priA 1169281..1171479 (+) 2199 WP_001324365.1 primosomal protein N' Machinery gene
  ABU997_RS07165 (ABU997_07170) cytR 1171635..1172660 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  ABU997_RS07170 (ABU997_07175) ftsN 1172752..1173711 (+) 960 WP_000068837.1 cell division protein FtsN -
  ABU997_RS07175 (ABU997_07180) hslV 1173804..1174334 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  ABU997_RS07180 (ABU997_07185) hslU 1174344..1175675 (+) 1332 WP_001293341.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81650.94 Da        Isoelectric Point: 9.0294

>NTDB_id=910982 ABU997_RS07160 WP_001324365.1 1169281..1171479(+) (priA) [Escherichia coli strain 10035210-2]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
VGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPMFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=910982 ABU997_RS07160 WP_001324365.1 1169281..1171479(+) (priA) [Escherichia coli strain 10035210-2]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCTGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGATTGTGGTATCAGTTAGCGATGCCAGCG
AACTGCCGCTCAATGAGCTAAAAGCGGTAGTCGAAGTGCTGGATAGTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTG
CTGCTATGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCACCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCGGCTTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGTGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACACCCGCACTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GTGGGGCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGTCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTAGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGATGTTCCCCGGCGTGCCCAT
TTCACGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTTTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGCCCGGTTCCGGCTCTGGCACCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATCTTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGTTGGATGTCGATCCCATCGAAGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451