Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SD437_RS12335 Genome accession   NZ_CP139565
Coordinates   2482085..2483155 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain NCCP     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2477085..2488155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD437_RS12285 - 2477144..2477728 (-) 585 WP_012117977.1 signal peptidase I -
  SD437_RS12290 tapA 2477700..2478371 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  SD437_RS12295 - 2478630..2478959 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  SD437_RS12300 - 2479000..2479179 (-) 180 WP_003153093.1 YqzE family protein -
  SD437_RS12305 comGG 2479236..2479613 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  SD437_RS12310 comGF 2479614..2480009 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  SD437_RS12315 comGE 2480023..2480337 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  SD437_RS12320 comGD 2480321..2480758 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  SD437_RS12325 comGC 2480748..2481056 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  SD437_RS12330 comGB 2481061..2482098 (-) 1038 WP_345912362.1 competence type IV pilus assembly protein ComGB Machinery gene
  SD437_RS12335 comGA 2482085..2483155 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  SD437_RS12340 - 2483348..2484298 (-) 951 WP_071181848.1 magnesium transporter CorA family protein -
  SD437_RS12345 - 2484444..2485745 (+) 1302 WP_071181849.1 hemolysin family protein -
  SD437_RS12350 - 2485783..2486613 (-) 831 WP_017418143.1 STAS domain-containing protein -
  SD437_RS12360 - 2486820..2487200 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  SD437_RS12365 - 2487437..2487682 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=910228 SD437_RS12335 WP_014418378.1 2482085..2483155(-) (comGA) [Bacillus velezensis strain NCCP]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=910228 SD437_RS12335 WP_014418378.1 2482085..2483155(-) (comGA) [Bacillus velezensis strain NCCP]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374