Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   SD437_RS12330 Genome accession   NZ_CP139565
Coordinates   2481061..2482098 (-) Length   345 a.a.
NCBI ID   WP_345912362.1    Uniprot ID   -
Organism   Bacillus velezensis strain NCCP     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476061..2487098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD437_RS12280 - 2476294..2477079 (-) 786 WP_017418136.1 TasA family protein -
  SD437_RS12285 - 2477144..2477728 (-) 585 WP_012117977.1 signal peptidase I -
  SD437_RS12290 tapA 2477700..2478371 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  SD437_RS12295 - 2478630..2478959 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  SD437_RS12300 - 2479000..2479179 (-) 180 WP_003153093.1 YqzE family protein -
  SD437_RS12305 comGG 2479236..2479613 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  SD437_RS12310 comGF 2479614..2480009 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  SD437_RS12315 comGE 2480023..2480337 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  SD437_RS12320 comGD 2480321..2480758 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  SD437_RS12325 comGC 2480748..2481056 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  SD437_RS12330 comGB 2481061..2482098 (-) 1038 WP_345912362.1 competence type IV pilus assembly protein ComGB Machinery gene
  SD437_RS12335 comGA 2482085..2483155 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  SD437_RS12340 - 2483348..2484298 (-) 951 WP_071181848.1 magnesium transporter CorA family protein -
  SD437_RS12345 - 2484444..2485745 (+) 1302 WP_071181849.1 hemolysin family protein -
  SD437_RS12350 - 2485783..2486613 (-) 831 WP_017418143.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39617.92 Da        Isoelectric Point: 9.6517

>NTDB_id=910227 SD437_RS12330 WP_345912362.1 2481061..2482098(-) (comGB) [Bacillus velezensis strain NCCP]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=910227 SD437_RS12330 WP_345912362.1 2481061..2482098(-) (comGB) [Bacillus velezensis strain NCCP]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GACGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.944

93.623

0.617